鉴定和定量蜂巢（中华蜜蜂巢脾）中的生物活性成分。
Identification and quantitation of bioactive components from honeycomb (Nidus Vespae).
机构信息
School of Chemical Engineering, Northwest University, 229 North TaiBai Road, Xi'an 710069, China.
School of Chemical Engineering, Northwest University, 229 North TaiBai Road, Xi'an 710069, China; College of Life Science and Technology, Xinjiang University, Urumqi 830046, China.
出版信息
Food Chem. 2020 Jun 1;314:126052. doi: 10.1016/j.foodchem.2019.126052. Epub 2019 Dec 17.
Honeycomb (Nidus Vespae) as an agri-food waste in bee product industry is in soaring demand for high-value utilization. This study is dedicated to investigate the physicochemical properties, chemical composition, and nutritional value of honeycomb by determination of physicochemical parameters, total phenolic and total flavonoid contents, antioxidant capacity in vitro, and bioactive components. By using ultra high-performance liquid chromatography-high resolution mass spectrometry (UHPLC-HRMS) method, a total of 76 bioactive components from hydro-ethanolic extracts of honeycomb (EHB) were tentatively identified, where the principal ones are polyphenols and fatty acids, which were further quantified by LC-MS and GC-MS, respectively. Moreover, antimicrobial activities test has been conducted, verifying that EHB can inhibit both Gram-negative (G) bacteria and Gram-positive (G+) bacteria, which is beneficial for the high-value utilization of honeycomb.
蜂巢（Nidus Vespae）作为蜂产品行业的农业食品废弃物，其高附加值的利用需求正在飙升。本研究旨在通过测定理化参数、总酚和总黄酮含量、体外抗氧化能力以及生物活性成分，来研究蜂巢的理化性质、化学成分和营养价值。采用超高效液相色谱-高分辨质谱联用（UHPLC-HRMS）方法，从蜂巢的水-乙醇提取物（EHB）中初步鉴定出 76 种生物活性成分，主要为多酚和脂肪酸，并分别通过 LC-MS 和 GC-MS 进行了定量分析。此外，还进行了抗菌活性测试，验证了 EHB 可以抑制革兰氏阴性（G）菌和革兰氏阳性（G+）菌，这有利于蜂巢的高价值利用。
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