芽中类黄酮提取的优化及其提取物的抗氧化和抗菌活性。
Optimization of Flavonoid Extraction from Buds, and the Antioxidant and Antibacterial Activities of the Extract.
机构信息
College of Life Engineering, Shenyang Institute of Technology, Fushun 113122, China.
Primorskaya State Academy of Agriculture, Ussuriisk 692510, Russia.
出版信息
Molecules. 2022 Sep 3;27(17):5695. doi: 10.3390/molecules27175695.
The present study was designed to evaluate the chemical extraction, chemical composition, and antioxidant and antibacterial properties of the total flavonoids in Willow Buds (TFW). We investigated the optimal extraction of TFW using response surface methodology (RSM). Chemical compounds were analyzed using Q-Orbitrap LC-MS/MS. The DPPH radical scavenging capacity, hydroxy radical inhibitory ability, and superoxide anion radical inhibitory ability were explored to determine the antioxidant properties of flavonoid extractions. The antibacterial effect was assessed via minimal inhibitory concentration. The results demonstrated that the optimal extraction conditions were an ethanol concentration of 50%, a time of 35 min, and a liquid/material ratio of 70:1 mL/g. Under these conditions, the yield of TFW was 7.57%. Eight flavonoids, a phenolic glycoside, and an alkaloid were enriched in the Willow Buds. The TFW exhibited significant antioxidant activity, with IC values of 0.18-0.24 mg/mL and antimicrobial activity against , , , and . TFW may be explored as potential and natural compounds in food and pharmacological applications.
本研究旨在评估柳芽总黄酮（TFW）的化学提取、化学成分、抗氧化和抗菌性能。我们采用响应面法（RSM）研究了 TFW 的最佳提取条件。采用 Q-Orbitrap LC-MS/MS 分析了化学化合物。通过 DPPH 自由基清除能力、羟基自由基抑制能力和超氧阴离子自由基抑制能力来探究黄酮提取物的抗氧化性能。通过最小抑菌浓度评估了抗菌效果。结果表明，最佳提取条件为乙醇浓度 50%、时间 35 分钟、液料比 70:1 mL/g。在此条件下，TFW 的产率为 7.57%。柳芽中富集了 8 种类黄酮、一种酚糖苷和一种生物碱。TFW 表现出显著的抗氧化活性，IC 值为 0.18-0.24 mg/mL，对 、 、 、 具有抗菌活性。TFW 可能作为食品和药理学应用中的潜在天然化合物进行探索。
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