Department of Microbiology, Key Lab of Microbiology for Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, China.
Experimental Biology Education Center, College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, China.
Curr Microbiol. 2020 Jun;77(6):1117-1124. doi: 10.1007/s00284-020-01889-5. Epub 2020 Jan 25.
A Gram-stain-negative, facultative aerobic, non-spore-forming, non-motile, non-flagellated, rod-shaped bacterium, designated strain NAU-18 was isolated from an oil-contaminated soil in China. Strain NAU-18 could grow at 10-42 °C (optimum, 30 °C), at pH 5.0-8.0 (optimum, 7.0) and in the presence of 0-2.0% (w/v) NaCl (optimum, 0.5% NaCl in R2A). The predominant fatty acids were Cω7c (71.2%) and Summed feature 2 (5.1%), representing 76.3% of the total fatty acids. The major respiratory quinones were Q9 and Q10. The DNA G + C content of strain NAU-18 was 61.4 mol% based on its draft genome sequence. Genome annotation of strain NAU-18 predicted the presence of 6668 genes, of which 6588 are coding proteins and 80 are RNA genes. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain NAU-18 was a member of the genus Rhizobium and showed 96.93% (with 93.2% coverage) and 96.81% (with 100% coverage) identities with those of Neorhizobium alkalisoli CCBAU 01393 and Rhizobium oryzicola ZYY136, respectively. In the phylogenetic analysis, strain NAU-18 and R. oryzicola ZYY136 are consistently placed in the same branch. Strain NAU-18 represents a novel species within the genus Rhizobium, for which the name Rhizobium terrae sp. nov. is proposed, with the type strain NAU-18 (=KCTC 62418 = CCTCC AB 2018075).
一株革兰氏阴性、兼性需氧、不产芽孢、不运动、无鞭毛的杆状细菌，被命名为 NAU-18 菌株，从中国受污染的土壤中分离得到。NAU-18 菌株可在 10-42°C（最适温度 30°C）、pH5.0-8.0（最适 pH7.0）和 0-2.0%（w/v）NaCl（最适 0.5%NaCl 在 R2A 中）的条件下生长。优势脂肪酸为 Cω7c（71.2%）和 Summed feature 2（5.1%），占总脂肪酸的 76.3%。主要呼吸醌为 Q9 和 Q10。菌株 NAU-18 的 DNA G+C 含量基于其草图基因组序列为 61.4mol%。基于 NAU-18 菌株的基因组注释，预测存在 6668 个基因，其中 6588 个是编码蛋白，80 个是 RNA 基因。基于 16S rRNA 基因序列的系统发育分析表明，NAU-18 菌株是根瘤菌属的一个成员，与 Neorhizobium alkalisoli CCBAU 01393 和 Rhizobium oryzicola ZYY136 的同源性分别为 96.93%（覆盖率为 93.2%）和 96.81%（覆盖率为 100%）。在系统发育分析中，NAU-18 菌株与 Rhizobium oryzicola ZYY136 始终处于同一分支。NAU-18 菌株代表根瘤菌属中的一个新种，命名为 Rhizobium terrae sp. nov.，其模式菌株为 NAU-18（=KCTC 62418= CCTCC AB 2018075）。
