Golubev Sergey, Rasterkovskaya Margarita, Sungurtseva Irina, Burov Andrey, Muratova Anna
Institute of Biochemistry and Physiology of Plants and Microorganisms, Saratov Scientific Centre of the Russian Academy of Sciences (IBPPM RAS), Saratov 410049, Russia.
Microorganisms. 2024 Aug 4;12(8):1586. doi: 10.3390/microorganisms12081586.
Pollutant degradation and heavy-metal resistance may be important features of the rhizobia, making them promising agents for environment cleanup biotechnology. The degradation of phenanthrene, a three-ring polycyclic aromatic hydrocarbon (PAH), by the rhizobial strain Rsf11 isolated from the oil-polluted rhizosphere of alfalfa and the influence of nickel ions on this process were studied. On the basis of whole-genome and polyphasic taxonomy, the bacterium Rsf11 represent a novel species of the genus , so the name sp. nov. was proposed. Analysis of phenanthrene degradation by the Rsf1 strain revealed 1-hydroxy-2-naphthoic acid as the key intermediate and the activity of two enzymes apparently involved in PAH degradation. It was also shown that the nickel resistance of Rsf11 was connected with the extracellular adsorption of metal by EPS. The joint presence of phenanthrene and nickel in the medium reduced the degradation of PAH by the microorganism, apparently due to the inhibition of microbial growth but not due to the inhibition of the activity of the PAH degradation enzymes. Genes potentially involved in PAH catabolism and nickel resistance were discovered in the microorganism studied. strain Rsf11 can be considered as a promising candidate for use in the bioremediation of mixed PAH-heavy-metal contamination.
污染物降解和重金属抗性可能是根瘤菌的重要特性，这使得它们成为环境净化生物技术中很有前景的媒介。研究了从苜蓿油污根际分离出的根瘤菌菌株Rsf11对菲（一种三环多环芳烃（PAH））的降解以及镍离子对该过程的影响。基于全基因组和多相分类学，细菌Rsf11代表了一个新的属种，因此提出了新物种的名称。对Rsf1菌株的菲降解分析表明，1-羟基-2-萘甲酸是关键中间体，并且有两种酶的活性明显参与PAH降解。还表明，Rsf11的镍抗性与EPS对金属的细胞外吸附有关。培养基中菲和镍的共同存在降低了微生物对PAH的降解，这显然是由于抑制了微生物生长，而不是由于抑制了PAH降解酶的活性。在研究的微生物中发现了可能参与PAH分解代谢和镍抗性的基因。菌株Rsf11可被视为用于多环芳烃-重金属混合污染生物修复的有前景的候选菌株。
