Rho GTP酶参与SCC-4口腔鳞状细胞癌细胞系细胞分化的调控：一项初步研究。
Rho GTPases are Involved on Regulation of Cytodifferentiation of SCC-4 Oral Squamous Cell Carcinoma Cell Line: A Preliminary Study.
作者信息
Pineiro Nanci M, Carneiro Anna Cecilia D M, Crema Virginia O
机构信息
Department of Structural Biology, Institute of Natural and Biological Sciences, Federal University of Triângulo Mineiro, Uberaba, Brazil.
出版信息
Asian Pac J Cancer Prev. 2020 Jan 1;21(1):3-6. doi: 10.31557/APJCP.2020.21.1.3.
OBJECTIVE
This study evaluated the involvement of Rho GTPases proteins in the regulation of cytodifferentiation of the SCC-4 human oral squamous cell carcinoma cell line.
METHODS
Cytokeratin and vimentin immunofluorescence and F-actin staining, assays were performed with control cells and Clostridium difficile 1, 2 and 4 μg/mL Toxin A (Rho GTPases inhibitor) treated SCC-4 cells on three-dimensional MatrigelTM for 24 h. Samples were analyzed by using confocal laser microscopy. Significances were p.
目的
本研究评估Rho GTPases蛋白在人口腔鳞状细胞癌细胞系SCC-4细胞分化调控中的作用。
方法
对对照细胞以及用1、2和4 μg/mL艰难梭菌毒素A（Rho GTPases抑制剂）处理的SCC-4细胞在三维基质胶上培养24小时，进行细胞角蛋白和波形蛋白免疫荧光以及F-肌动蛋白染色检测。使用共聚焦激光显微镜对样本进行分析。显著性为p。
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