Rho GTPases are Involved on Regulation of Cytodifferentiation of SCC-4 Oral Squamous Cell Carcinoma Cell Line: A Preliminary Study.

作者信息

Pineiro Nanci M, Carneiro Anna Cecilia D M, Crema Virginia O

机构信息

Department of Structural Biology, Institute of Natural and Biological Sciences, Federal University of Triângulo Mineiro, Uberaba, Brazil.

出版信息

Asian Pac J Cancer Prev. 2020 Jan 1;21(1):3-6. doi: 10.31557/APJCP.2020.21.1.3.

DOI:10.31557/APJCP.2020.21.1.3

PMID:31983155

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7294035/

Abstract

OBJECTIVE

This study evaluated the involvement of Rho GTPases proteins in the regulation of cytodifferentiation of the SCC-4 human oral squamous cell carcinoma cell line.

METHODS

Cytokeratin and vimentin immunofluorescence and F-actin staining, assays were performed with control cells and Clostridium difficile 1, 2 and 4 μg/mL Toxin A (Rho GTPases inhibitor) treated SCC-4 cells on three-dimensional MatrigelTM for 24 h. Samples were analyzed by using confocal laser microscopy. Significances were p.

摘要