Souza Augusto Monteiro de, Lopes Otávio Sérgio, Liberato Andressa de Lima, Oliveira Paulo Junior Ribeiro de, Herrero Sylvia Satomi Takeno, Nascimento Agnaldo Luiz do, Longui Carlos Alberto, Carvalho Filho Ivan Rodrigues de, Soares Leonardo Ferreira, Silva Renally Barbosa da, Burbano Rommel Rodriguez, Delatorre Plínio, Lima Eleonidas Moura
Laboratory of Structural Molecular Biology and Oncogenetics - LBMEO, Federal University of Paraiba, João Pessoa - PB, Brazil.
Postgraduate Program in Cellular and Molecular Biology, Federal University of Paraiba, João Pessoa - PB, Brazil.
Asian Pac J Cancer Prev. 2020 Jan 1;21(1):25-29. doi: 10.31557/APJCP.2020.21.1.25.
Perform genotyping of SNPs in the promoter region of the SMO gene in BCC samples from patients from northeastern Brazil, and to determine if there is an association of these SNPs of the gene in question with the susceptibility to the development of the BCC.
100 samples of paraffined tissue from patients with histopathological diagnosis of BCC and 100 control samples were analyzed for each polymorphism by a newly developed genotyping method, the Dideoxy Single Allele Specific - PCR. The software Bioestat - version 5.3 and Haploview 4.2 were used for the statistical analysis. For all tests a P-value.
对来自巴西东北部患者的基底细胞癌（BCC）样本中SMO基因启动子区域的单核苷酸多态性（SNP）进行基因分型，并确定该基因的这些SNP与BCC发生易感性之间是否存在关联。
采用新开发的基因分型方法——双脱氧单等位基因特异性PCR，对100例经组织病理学诊断为BCC的患者石蜡组织样本和100例对照样本的每种多态性进行分析。使用Bioestat 5.3软件和Haploview 4.2进行统计分析。所有检验均采用P值。
