Mirzaiian Elham, Tabatabaei Ghods Zahra Sadat, Tavangar Seyed Mohammad, Emami Binesh, Oraie Maryam, Safyari Roya, Saffar Hiva
Department of Pathology, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran.
Anatomical and Clinical Pathologist, Farvardin Laboratory, Tehran, Iran.
Asian Pac J Cancer Prev. 2020 Jan 1;21(1):63-66. doi: 10.31557/APJCP.2020.21.1.63.
Mitotic activity index is considered as the most important grading component to predict prognosis in invasive breast carcinoma. But it is believed that it is also the cause of discordance in grade estimation based on Bloom-Richardson system. Thus, reproducible methods such as immunohistochemistry (IHC) based analysis methods appears to be of great value in facilitating mitotic count.
In the present study, we examined the utility of Phosphohistone H3 by IHC in various grades of breast carcinoma and compared it with traditional mitotic count by hematoxylin and eosin (H and E) staining and probable changes in tumor grading.
Total 90 cases of invasive breast carcinoma were evaluated. Mean mitotic count were 8.6 and 6.4/10HPF in IHC and HandE groups, respectively. Although , mean average count was higher by IHC method , good correlation was observed(R=0.914). Using PHH3 IHC, two out of 33 cases of grade I tumors were upgraded in to grade II and three cases of grade II were upgraded in to grade III. None of the tumors were down graded.
Similar to some other previous studies, we found PHH3 a robust sensitive and practical marker for mitotic count in breast carcinoma. Especially it is helpful to identify the most proliferating area. However, further studies are required to confirm the superiority of this biomarker for including in grading system.
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有丝分裂活性指数被认为是预测浸润性乳腺癌预后的最重要分级组成部分。但据信它也是基于布鲁姆-理查森系统的分级评估中出现不一致的原因。因此，诸如基于免疫组织化学（IHC）的分析方法等可重复的方法在促进有丝分裂计数方面似乎具有很大价值。
在本研究中，我们检测了免疫组织化学法检测磷酸化组蛋白H3在不同分级乳腺癌中的应用，并将其与苏木精和伊红（H&E）染色的传统有丝分裂计数以及肿瘤分级可能的变化进行了比较。
共评估了90例浸润性乳腺癌病例。免疫组织化学组和苏木精和伊红染色组的平均有丝分裂计数分别为8.6和6.4/10高倍视野。虽然免疫组织化学法的平均计数较高，但观察到良好的相关性（R = 0.914）。使用磷酸化组蛋白H3免疫组织化学法，33例I级肿瘤中有2例升级为II级，3例II级肿瘤升级为III级。没有肿瘤降级。
与其他一些先前的研究相似，我们发现磷酸化组蛋白H3是乳腺癌有丝分裂计数的一种可靠、敏感且实用的标志物。特别是它有助于识别增殖最活跃的区域。然而，需要进一步研究来证实这种生物标志物在纳入分级系统方面的优越性。
