Harris Rachel A, Leaptrot Katrina L, May Jody C, McLean John A
Department of Chemistry, Center for Innovative Technology, Vanderbilt Institute of Chemical Biology, Vanderbilt Institute for Integrative Biosystems Research and Education, Vanderbilt-Ingram Cancer Center, Vanderbilt University, Nashville Tennessee 37235, United States.
Trends Analyt Chem. 2019 Jul;116:316-323. doi: 10.1016/j.trac.2019.03.031. Epub 2019 Apr 6.
The growth of lipidomics and the high isomeric complexity of the lipidome has revealed a need for analytical techniques capable of structurally characterizing lipids with a high degree of specificity. Lipids are morphologically diverse molecules that can exist as any one of a large number of isomeric species, and as such are often indistinguishable by mass spectrometry without a complementary separation method. Recent developments in the field of lipidomics aim to address these challenges by utilizing a combination of multiple analytical techniques which are selective to lipid primary structure. This review summarizes two emerging strategies for lipidomic analysis, namely, ion mobility-mass spectrometry and ion fragmentation via ozonolysis.
脂质组学的发展以及脂质组高度的异构体复杂性表明，需要能够以高度特异性对脂质进行结构表征的分析技术。脂质是形态多样的分子，可以以大量异构体形式中的任何一种存在，因此在没有互补分离方法的情况下，质谱往往无法区分它们。脂质组学领域的最新进展旨在通过结合多种对脂质一级结构具有选择性的分析技术来应对这些挑战。本综述总结了脂质组学分析的两种新兴策略，即离子淌度-质谱联用和臭氧分解离子碎裂法。
