Abbasi Naser, Azizpour Yosra, Azizi Monireh, Karimi Elahe, Aidy Ali, Asadollahi Khairollah
Biotechnology and Medicinal Plants Researches Centre, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran.
Department of Pharmacology, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran.
J Stem Cells Regen Med. 2019 Dec 24;15(2):18-23. doi: 10.46582/jsrm.1502006. eCollection 2019.
Mumie, as an inorganic and semi-solid herbal substance, could be obtained from crevice caves and is used for bone diseases in traditional medicine. This study investigated the effects of this substance on the expression of bone alkaline phosphatase (BALP) enzyme as well as proliferation and mortality rates of MG63 human osteoblast-like cells. The MG63 cells were cultured and the effect of 100, 200 and 300 μg/ml of mumie extract on cell viability were compared with zoledronic acid and estradiol valerate as positive controls, as well as with MG63 cells alone as the negative control group. The activity rate of the BALP enzyme was also assessed. During 48 hours of the study period, the concentrations of 100 and 200μg/ml of mumie extract increased the proliferation rate and decreased the mortality rate of MG63 cells significantly; however, the concentration of 300μg/ml decreased the proliferation rate and increased the mortality rate of the cells. Also, BALP enzyme expression was slightly affected by 100 and 200 μg/ml of mumie extract whilst it was significantly decreased by the concentration of 300 μg/ml. This study showed that mumie extract has an increasing effect on proliferation rate and a decreasing effect on the mortality rate of osteoblast cells in low concentrations; however, the higher concentrations of this substance could be toxic and effect inversely.
木乃伊粉作为一种无机半固体草药物质，可从裂隙洞穴中获取，在传统医学中用于治疗骨骼疾病。本研究调查了该物质对骨碱性磷酸酶（BALP）表达以及MG63人成骨样细胞增殖率和死亡率的影响。培养MG63细胞，并将100、200和300μg/ml的木乃伊粉提取物对细胞活力的影响与作为阳性对照的唑来膦酸和戊酸雌二醇以及仅作为阴性对照组的MG63细胞进行比较。还评估了BALP酶的活性率。在48小时的研究期间，100和200μg/ml的木乃伊粉提取物浓度显著提高了MG63细胞的增殖率并降低了死亡率；然而，300μg/ml的浓度降低了细胞的增殖率并提高了死亡率。此外，100和200μg/ml的木乃伊粉提取物对BALP酶表达有轻微影响，而300μg/ml的浓度则使其显著降低。本研究表明，低浓度的木乃伊粉提取物对成骨细胞的增殖率有增加作用，对死亡率有降低作用；然而，该物质的高浓度可能具有毒性，作用相反。
