Kanhere Aditi, Lin Guangyun, Jiang Hongrui
Department of Electrical and Computer Engineering, University of Wisconsin, Madison, WI 53706 USA.
Department of Electrical and Computer Engineering, the Department of Biomedical Engineering, the Materials Science Program and the McPherson Eye Research Institute, University of Wisconsin, Madison WI 53706 USA.
J Microelectromech Syst. 2016 Apr;25(2):304-310. doi: 10.1109/jmems.2016.2518922. Epub 2016 Feb 3.
This work presents the use of a tunable-focus thermo-responsive hydrogel based liquid lens in combination with an objective lens to achieve remote axial focusing in a conventional microscopy. The goal of this design is to eliminate image distortion due to sample vibrations caused by mechanical stage scanning. This approach reduces the mechanical complexity and power consumption due to the use of electrically tunable lenses while achieving a two-fold increase in the axial scanning range. The merits of the proposed design were demonstrated by characterizing a customized microscope system over a scanning range of 1700 μm. A lateral resolution of 2 μm was obtained consistently throughout the scanning range. Healthy Sf21 insect cells imaging was used to verify the depth scanning ability and the resolution of our remote focusing microscope system.
这项工作展示了一种基于可调焦热响应水凝胶的液体透镜与物镜相结合的应用，以在传统显微镜中实现远程轴向聚焦。该设计的目标是消除由于机械载物台扫描引起的样品振动导致的图像失真。这种方法由于使用了电可调透镜，降低了机械复杂性和功耗，同时轴向扫描范围增加了两倍。通过在1700μm的扫描范围内对定制显微镜系统进行表征，证明了所提出设计的优点。在整个扫描范围内始终获得了2μm的横向分辨率。使用健康的Sf21昆虫细胞成像来验证我们的远程聚焦显微镜系统的深度扫描能力和分辨率。
