Said Kamaleldin B, Aljarbou Ahmed N, Alorainy Mohamed S, Saeed Elhassan M A, Hassan Khalid M
Department of Pathology and Microbiology, College of Medicine, University of Hail, Hail, Saudi Arabia.
Dean, Al Ghad International Colleges for Applied Medical Sciences, Qassim, Saudi Arabia.
Int J Health Sci (Qassim). 2020 Jan-Feb;14(1):9-19.
has become an important pathogen in hospitals worldwide. Despite its differentiation into human and animal lineages, common methods are used for genotyping. While these methods are useful, they are based on the stable genome, and hence, are insensitive to host-specific subtyping. The objectives of this study were to investigate the repeat-domain of the Clumping-Factor A gene ( R) as an objective and adaptation-sensitive approach.
We have used 113 isolates for susceptibility testing and genotyping by polymerase chain reaction amplification of the R regions. Of these, 105 were from King Fahad Specialist Hospital, Buraidah and eight were published sequences used as references. Isolates were further confirmed as by the commercial Kits. Amplicon sizes were measured and the number of the 18-bp-repeating-units in each isolate was determined against that of methicillin-resistant COL (MRSA) sequence.
Results showed that all 42 nasal screening isolates (100%) and all but six isolates from clinical specimens were MRSA with 37% of the former and 50% of the latter isolates showing community-acquired-MRSA susceptibility patterns. -R analysis grouped 113 isolates into 14 repeat-genotypes. The two dominant types, D and X, represented the long- and short -R types found in humans and animals, respectively. Linezolid, rifampicin, and vancomycin were the drugs of choice.
-R was useful in rapid genotyping and implied host-specific phenotypic properties of the . It has been recommended that the approach used in regional laboratories for uniform strain-profiling. Future work will show more insights into the gene content and origins of clones .
已成为全球医院中的一种重要病原体。尽管它可分为人类和动物谱系，但常用的基因分型方法却是相同的。虽然这些方法有用，但它们基于稳定的基因组，因此对宿主特异性亚型分型不敏感。本研究的目的是调查凝聚因子A基因（R）的重复结构域，作为一种客观且对适应性敏感的方法。
我们使用了113株分离株进行药敏试验，并通过聚合酶链反应扩增R区域进行基因分型。其中，105株来自布赖代法赫德国王专科医院，8株是已发表的用作参考的序列。分离株通过商业试剂盒进一步确认为[具体细菌名称]。测量扩增子大小，并根据耐甲氧西林金黄色葡萄球菌COL（MRSA）序列确定每个分离株中18碱基对重复单元的数量。
结果显示，所有42株鼻腔筛查分离株（100%）以及除6株临床标本分离株外的所有分离株均为MRSA，前者37%和后者50%的分离株表现出社区获得性MRSA药敏模式。R分析将113株分离株分为14种重复基因型。两种主要类型，D型和X型，分别代表在人类和动物中发现的长R型和短R型。利奈唑胺、利福平和万古霉素是首选药物。
R分析在快速基因分型中很有用，并暗示了[具体细菌名称]的宿主特异性表型特性。建议区域实验室采用该方法进行统一的菌株分析。未来的工作将对克隆的基因内容和起源有更多深入了解。
