Hubei International Scientific and Technological Cooperation Base of Traditional Fermented Foods, Key Laboratory of Environment Correlative Dietology, College of Food Science and Technology , Huazhong Agricultural University , Wuhan , Hubei Province 430070 , China.
J Agric Food Chem. 2020 Feb 19;68(7):2146-2154. doi: 10.1021/acs.jafc.9b07916. Epub 2020 Feb 7.
Flavonoids are valuable natural products widely used in human health and nutrition applications. Engineering microbial consortia to express complex flavonoid biosynthetic pathways is a promising approach for flavonoid production. In this study, the entire flavonoid biosynthetic pathway was split into two independent pathways, each of which was contained in separate cells. The first cell type, sNAR5, which was genetically engineered to express the naringenin biosynthetic pathway, produced 144.1 mg/L naringenin. The second cell type was genetically modified with the heterologous naringenin-to-delphinidin pathway. A coculture produced a delphinidin titer, significantly higher than that produced in a monoculture of strain sDPD2, harboring the entire pathway. Furthermore, we successfully employed this coculture platform for the production of 3 flavonols and 2 anthocyanidins in flask-scale culture. This coculture platform paves the way for the development of an economical and efficient process for microbial flavonoid production.
类黄酮是一种有价值的天然产物，广泛应用于人类健康和营养领域。利用工程微生物群落来表达复杂的类黄酮生物合成途径是一种很有前途的类黄酮生产方法。在本研究中，将整个类黄酮生物合成途径分为两条独立的途径，每条途径都包含在单独的细胞中。第一个细胞类型 sNAR5 经过基因工程改造，表达了柚皮素生物合成途径，可生产 144.1mg/L 的柚皮素。第二个细胞类型经过异源柚皮素-飞燕草素途径的修饰。共培养产生的飞燕草素浓度明显高于含有整个途径的单培养菌株 sDPD2。此外，我们还成功地在摇瓶培养中利用该共培养平台生产了 3 种类黄酮醇和 2 种花青素。该共培养平台为开发经济高效的微生物类黄酮生产工艺铺平了道路。
