Inal Melissa Ana, Banzai Kota, Kamiyama Daichi
Department of Cellular Biology, University of Georgia.
Department of Cellular Biology, University of Georgia;
J Vis Exp. 2020 Jan 12(155). doi: 10.3791/60716.
We describe a technique for retrograde labeling of motor neurons in Drosophila. We use an oil-dissolved lipophilic dye and deliver a small droplet to an embryonic fillet preparation by a microinjector. Each motor neuron whose membrane is contacted by the droplet can then be rapidly labeled. Individual motor neurons are continuously labeled, enabling fine structural details to be clearly visualized. Given that lipophilic dyes come in various colors, the technique also provides a means to get adjacent neurons labeled in multicolor. This tracing technique is therefore useful for studying neuronal morphogenesis and synaptic connectivity in the motor neuron system of Drosophila.
我们描述了一种在果蝇中对运动神经元进行逆行标记的技术。我们使用一种油溶性亲脂性染料，并通过微量注射器将一小滴染料注入胚胎去内脏标本中。然后，每个其细胞膜与染料滴接触的运动神经元都能被快速标记。单个运动神经元被持续标记，从而能够清晰地观察到精细的结构细节。鉴于亲脂性染料有多种颜色，该技术还提供了一种对相邻神经元进行多色标记的方法。因此，这种追踪技术对于研究果蝇运动神经元系统中的神经元形态发生和突触连接是有用的。
