Ali Ahmed, Abouleila Yasmine, Germond Arno
Biodynamics Research Center (BDR), RIKEN; Research Center, Misr International University.
Biodynamics Research Center (BDR), RIKEN;
J Vis Exp. 2020 Jan 9(155). doi: 10.3791/60449.
Cells are known to be inherently heterogeneous in their responses to drugs. Therefore, it is essential that single-cell heterogeneity is accounted for in drug discovery studies. This can be achieved by accurately measuring the plethora of cellular interactions between a cell and drug at the single-cell level (i.e., drug uptake, metabolism, and effect). This paper describes a single-cell Raman spectroscopy and mass spectrometry (MS) platform to monitor metabolic changes of cells in response to drugs. Using this platform, metabolic changes in response to the drug can be measured by Raman spectroscopy, while the drug and its metabolite can be quantified using mass spectrometry in the same cell. The results suggest that it is possible to access information about drug uptake, metabolism, and response at a single-cell level.
众所周知，细胞对药物的反应本质上是异质的。因此，在药物发现研究中考虑单细胞异质性至关重要。这可以通过在单细胞水平上准确测量细胞与药物之间大量的细胞相互作用来实现（即药物摄取、代谢和效应）。本文描述了一种用于监测细胞对药物反应的代谢变化的单细胞拉曼光谱和质谱（MS）平台。使用该平台，可以通过拉曼光谱测量细胞对药物反应的代谢变化，而药物及其代谢物可以在同一细胞中使用质谱进行定量。结果表明，在单细胞水平上获取有关药物摄取、代谢和反应的信息是可能的。
