Department of Otorhinolaryngology, The Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu 213000, P.R. China.
Int J Mol Med. 2020 Mar;45(3):836-846. doi: 10.3892/ijmm.2020.4467. Epub 2020 Jan 15.
Circular RNAs have been reported to play a vital role in the development and progression of various types of cancer. However, the underlying molecular role of circular RNA CTDP1 (circCTDP1) in the tumorigenesis of nasopharyngeal carcinoma (NPC) remains unknown. In the present study, circCTDP1 expression was found to be markedly upregulated in NPC tissues and cell lines (SUNE1, SUNE2 and 6‑10B cell lines). Knockdown of circCTDP1 resulted in inhibition of proliferation, migration and invasion, and promoted apoptosis of NPC cells. Moreover, circCTDP1 directly interacted with microRNA (miR)‑320b based on bioinformatics prediction and dual luciferase assay, and transfection with an miR‑320b inhibitor reversed the effects of circCTDP1 knockdown on NPC cells. Furthermore, circCTDP1/miR‑320b promoted NPC progression by regulating the expression of homeobox A10 (HOXA10). In addition, it was demonstrated that HOXA10 may exert its oncogenic role in NPC by regulating the expression of transforming growth factor β2 (TGFβ2). Taken together, these results revealed a novel regulatory mechanism, which may provide an improved understanding of NPC tumorigenesis and be useful in the development of potential targets for NPC therapy.
环状 RNA 已被报道在多种类型癌症的发生和发展中发挥重要作用。然而，环状 RNA CTDP1（circCTDP1）在鼻咽癌（NPC）发生中的潜在分子作用仍不清楚。本研究发现，circCTDP1 在 NPC 组织和细胞系（SUNE1、SUNE2 和 6-10B 细胞系）中表达明显上调。circCTDP1 敲低导致 NPC 细胞增殖、迁移和侵袭受到抑制，并促进细胞凋亡。此外，基于生物信息学预测和双荧光素酶报告基因实验证实，circCTDP1 可直接与 microRNA（miR）-320b 相互作用，而 miR-320b 抑制剂的转染可逆转 circCTDP1 敲低对 NPC 细胞的影响。此外，circCTDP1/miR-320b 通过调节同源盒 A10（HOXA10）的表达促进 NPC 的进展。此外，研究表明 HOXA10 可能通过调节转化生长因子 β2（TGFβ2）的表达在 NPC 中发挥致癌作用。综上所述，这些结果揭示了一种新的调控机制，可能有助于深入了解 NPC 的肿瘤发生机制，并为 NPC 治疗的潜在靶点的开发提供参考。
