Jin Chengluo, Shi Linmei, Li Zhexun, Liu Wei, Zhao Bai, Qiu Yu, Zhao Yakun, Li Kunlun, Li Yifei, Zhu Qingguo
Department of Urology, The Second Affiliate Hospital, Harbin Medical University Harbin, China.
College of Public Health, Harbin Medical University Harbin, China.
Am J Transl Res. 2019 Aug 15;11(8):4935-4945. eCollection 2019.
Circular RNAs (circRNAs) belong to non-coding RNAs and are known as key regulators in gene regulation. CircRNAs involve in the various biological processes of cancer. However, the functions of circRNAs in renal cell carcinoma (RCC) are still not clear. In this study, the circRNA expression profile was performed in the RCC tissues by microarray. There were 35 significantly expressed circRNAs with more than 5 folds from microarray analysis. Hsa_circ_0039569 (circ_0039569) was verified to be up-regulated in RCC and cells compared with the controls by real time RT-PCR. The assays of cellular functions showed that circ_0039569 down-regulation suppressed the proliferation and metastasis of RCC cells. The molecular mechanism of circ_0039569 in RCC cells showed that circ_0039569 promoted RCC progression by up-regulating CCL22 expression via down-regulating miR-34a-5p. Taken together, the study indicated that circ_0039569 played an important role in RCC cell survival and metastasis, which suggested that an oncogenic role of circ_0039569 in RCC progression.
环状RNA（circRNAs）属于非编码RNA，是基因调控中的关键调节因子。circRNAs参与癌症的各种生物学过程。然而，circRNAs在肾细胞癌（RCC）中的功能仍不清楚。在本研究中，通过微阵列对RCC组织进行了circRNA表达谱分析。微阵列分析显示有35种显著表达的circRNAs，其表达倍数超过5倍。通过实时RT-PCR验证，与对照相比，hsa_circ_0039569（circ_0039569）在RCC组织和细胞中上调。细胞功能检测表明，circ_0039569下调可抑制RCC细胞的增殖和转移。circ_0039569在RCC细胞中的分子机制表明，circ_0039569通过下调miR-34a-5p上调CCL22表达来促进RCC进展。综上所述，该研究表明circ_0039569在RCC细胞存活和转移中起重要作用，提示circ_0039569在RCC进展中具有致癌作用。
