Department of Experimental Management Center, Henan Institute of Science and Technology, Xinxiang, China.
Department of Pharmacy, Xinxiang Central Hospital, Xinxiang, China.
J Cell Physiol. 2020 Oct;235(10):6515-6527. doi: 10.1002/jcp.29459. Epub 2020 Jan 27.
The incidence and mortality rate of hepatocellular carcinoma (HCC) nowadays is still at high levels. The regulatory roles of pseudogene in cancers have been gradually recognized in recent years. However, comprehensive investigation of abnormally expressed pseudogene and related mechanisms in HCC remains lacking. GSE124535 dataset was used to identify differentially expressed pseudogenes in HCC tissues compared with normal tissues. Prognostic value of these differentially expressed pseudogenes was analyzed at GEPIA. StarBase used to analyze microRNAs (miRNAs) can bind with pseudogene, while the targets for these miRNAs were analyzed at miRTarBase. Protein-protein interaction (PPI) network was then established for miRNA targets, after that hub genes were selected. Expression correlation of pseudogene and hub genes was analyzed at StarBase. In total, 16 upregulated and 17 downregulated pseudogenes were identified. Pseudogene HSPB1P1 was identified abnormally expressed in 20 types of human cancers and could be used as an indicator for poorer overall survival of patients with HCC. Functional analyses showed that HSPB1P1 was strongly correlated with signaling pathways related to cancer progression. Further studied revealed that HSPB1P1 could direct regulate the EZH2 expression in HCC. In summary, our study indicated that HSPB1P1 was a predictor for poorer overall survival of patients with HCC and may be potential therapeutic target against HCC.
如今，肝细胞癌 (HCC) 的发病率和死亡率仍然居高不下。近年来，人们逐渐认识到假基因在癌症中的调控作用。然而，对于 HCC 中异常表达的假基因及其相关机制的全面研究仍有待进一步探索。本研究使用 GSE124535 数据集鉴定 HCC 组织与正常组织相比差异表达的假基因。在 GEPIA 中分析这些差异表达假基因的预后价值。使用 StarBase 分析可以与假基因结合的 microRNAs (miRNAs)，同时在 miRTarBase 中分析这些 miRNAs 的靶基因。然后建立 miRNA 靶基因的蛋白质-蛋白质相互作用 (PPI) 网络，之后选择枢纽基因。在 StarBase 中分析假基因和枢纽基因的表达相关性。总共鉴定出 16 个上调和 17 个下调的假基因。假基因 HSPB1P1 在 20 种人类癌症中异常表达，可作为 HCC 患者总体生存率较差的指标。功能分析表明，HSPB1P1 与癌症进展相关的信号通路密切相关。进一步研究表明，HSPB1P1 可以直接调节 HCC 中的 EZH2 表达。综上所述，本研究表明 HSPB1P1 是 HCC 患者总体生存率较差的预测因子，可能是 HCC 潜在的治疗靶点。
