Gurumurthy Bhuvaneswari, Tucci Michelle A, Fan Lir-Wan, Benghuzzi Hamed A, Pal Pallabi, Bidwell Gene L, Salazar Marocho Susana M, Cason Zelma, Gordy David, Janorkar Amol V
Department of Biomedical Materials Science, School of Dentistry, University of Mississippi Medical Center, Jackson, MS, 39216, USA.
Department of Anesthesiology, University of Mississippi Medical Center, Jackson, MS, 39216, USA.
Adv Healthc Mater. 2020 Feb;9(4):e1901385. doi: 10.1002/adhm.201901385. Epub 2020 Jan 27.
The goals of this study are to evaluate the ability of the multicomponent collagen-elastin-like polypeptide (ELP)-Bioglass scaffolds to support osteogenesis of rat mesenchymal stem cells (rMSCs), demonstrate in vivo biocompatibility by subcutaneous implantation in Sprague-Dawley rats, monitor degradation noninvasively, and finally assess the scaffold's ability in healing critical-sized cranial bone defects. The collagen-ELP-Bioglass scaffold supports the in vitro osteogenic differentiation of rMSCs over a 3 week culture period. The cellular (rMSC-containing) or acellular scaffolds implanted in the subcutaneous pockets of rats do not cause any local or systemic toxic effects or tumors. The real-time monitoring of the fluorescently labeled scaffolds by IVIS reveals that the scaffolds remain at the site of implantation for up to three weeks, during which they degrade gradually. Micro-CT analysis shows that the bilateral cranial critical-sized defects created in rats lead to greater bone regeneration when filled with cellular scaffolds. Bone mineral density and bone microarchitectural parameters are comparable among different scaffold groups, but the histological analysis reveals increased formation of high-quality mature bone in the cellular group, while the acellular group has immature bone and organized connective tissue. These results suggest that the rMSC-seeded collagen-ELP-Bioglass composite scaffolds can aid in better bone healing process.
本研究的目标是评估多组分胶原 - 弹性蛋白样多肽（ELP）-生物玻璃支架支持大鼠间充质干细胞（rMSCs）成骨的能力，通过在Sprague-Dawley大鼠皮下植入来证明其体内生物相容性，无创监测其降解情况，并最终评估该支架修复临界尺寸颅骨缺损的能力。在为期3周的培养期内，胶原 - ELP - 生物玻璃支架支持rMSCs的体外成骨分化。植入大鼠皮下囊袋的含细胞（含rMSC）或无细胞支架不会引起任何局部或全身毒性作用或肿瘤。通过IVIS对荧光标记支架进行实时监测发现，支架在植入部位可保留长达三周，在此期间它们会逐渐降解。显微CT分析表明，在大鼠中创建的双侧颅骨临界尺寸缺损在填充含细胞支架时会导致更大程度的骨再生。不同支架组之间的骨密度和骨微结构参数具有可比性，但组织学分析显示，含细胞组中高质量成熟骨的形成增加，而无细胞组则有未成熟骨和有组织的结缔组织。这些结果表明，接种rMSC的胶原 - ELP - 生物玻璃复合支架有助于更好地促进骨愈合过程。
