Andraszek Katarzyna, Banaszewska Dorota, Szeleszczuk Olga, Niedbała Piotr, Kuchta-Gładysz Marta
Department of Bioengineering and Animal Sciences, Siedlce University of Natural Sciences and Humanities, Siedlce, Poland.
Department of Animal Reproduction, Anatomy and Genomics, University of Agriculture in Krakow, Kraków, Poland.
Reprod Domest Anim. 2020 Apr;55(4):515-522. doi: 10.1111/rda.13646. Epub 2020 Feb 12.
Sperm cells isolated from the tail of the epididymis and from the semen of the same individuals were analysed. The use of silver nitrate to stain sperm cells isolated from the tail of the epididymis made it possible to identify structures that were not visible in the sperm from semen. Silver nitrate very clearly distinguished the acrosomal and distal parts of the sperm head. Following silver nitrate staining, the sperm isolated from the tail of the epididymis were characterized by dark 'collars' in the distal part of the head. These 'collars' are not visible in the sperm cells isolated from semen. The results of the study indicate differences in the dimensions of sperm isolated from the tail of the epididymis and sperm in semen. Sperm isolated from the tail of the epididymis had smaller heads, despite their longer length, and had longer midpieces and tails than ejaculate sperm. Silver nitrate staining is a simple and fast technique. Silver nitrate makes it possible to identify the acrosome and post-acrosomal region of the sperm head and to clearly identify the midpiece. Therefore, it can be successfully used to supplement routine techniques for evaluating sperm morphology or as an independent technique.
对从附睾尾部和同一个体精液中分离出的精子细胞进行了分析。使用硝酸银对从附睾尾部分离出的精子细胞进行染色，使得识别精液中精子不可见的结构成为可能。硝酸银能非常清晰地区分精子头部的顶体和远端部分。硝酸银染色后，从附睾尾部分离出的精子头部远端有深色的“环”。这些“环”在从精液中分离出的精子细胞中不可见。研究结果表明，从附睾尾部分离出的精子与精液中的精子在尺寸上存在差异。从附睾尾部分离出的精子头部较小，尽管其长度较长，并且与射精精子相比，其线粒体鞘和尾部更长。硝酸银染色是一种简单快速的技术。硝酸银能够识别精子头部的顶体和顶体后区域，并清晰地识别线粒体鞘。因此，它可以成功地用于补充评估精子形态的常规技术或作为一种独立技术。
