College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, 310058, China.
Zhijiangnan Think Tank, Zhejiang Institute of Science and Technology Information, Hangzhou, 310006, China.
Anal Chim Acta. 2020 Feb 22;1099:1-15. doi: 10.1016/j.aca.2019.11.056. Epub 2019 Nov 26.
Nucleic acid-based techniques could achieve highly sensitive detection by amplifying template molecules to millions of folds. It has been one of the most valued analytical methods and is applied in many detection fields, such as diagnosis of infectious diseases, food safety assurance and so on. Nucleic acid-based techniques consist of three steps: nucleic acid extraction, amplification, and product detection. Among them, the detection step plays a vital role because it shows the results directly. As the trend of detection is simple, rapid and instrument-free, it is of necessity to carry out visual detection, where the result read-out could be visible and distinguished by the naked eye. In this critical review, advanced visual detection methods are summarized and discussed in detail, aiming to promote the potential application in on-site detection.
基于核酸的技术可以通过将模板分子扩增到数百万倍来实现高度敏感的检测。它是最有价值的分析方法之一，应用于许多检测领域，如传染病的诊断、食品安全保障等。基于核酸的技术包括三个步骤：核酸提取、扩增和产物检测。其中，检测步骤起着至关重要的作用，因为它直接显示结果。随着检测趋势朝着简单、快速和无仪器化的方向发展，有必要进行可视化检测，结果可以通过肉眼进行可见和区分。在这篇重要的综述中，详细总结和讨论了先进的可视化检测方法，旨在促进其在现场检测中的潜在应用。
