Li Xiang-Ling, Zhao Lei, Wang Zi-Heng, Song Tian-Shun, Guo Ting, Xie Jing Jing
State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing Tech University, Nanjing, 211816, People's Republic of China.
College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, 211816, People's Republic of China.
Mikrochim Acta. 2024 Feb 9;191(3):127. doi: 10.1007/s00604-024-06204-8.
A one-target-many-trigger signal model sensing strategy is proposed for quickly, sensitive and on-site detection of the environmental pollutant p-aminophenol (PAP) by use of a commercial personal glucose meter (PGM) for signal readout with the core-shell "loading-type" nanomaterial MSNs@MnO as amplifiable nanoprobes. In this design, the mesoporous silica nanoparticles (MSNs) nanocontainer with entrapped signal molecule glucose is coated with redoxable manganese dioxide (MnO) nanosheets to form the amplifiable nanoprobes (Glu-MSNs@MnO). When encountered with PAP, the redox reaction between the MnO and PAP can induce the degradation of the outer layer of MSNs@MnO, liberating multiple copies of the loaded glucose to light up the PGM signal. Owing to the high loading capability of nanocarriers, a "one-to-many" relationship exists between the target and the signal molecule glucose, which can generate adequate signal outputs to achieve the requirement of on-site determination of environmental pollutants. Taking advantage of this amplification mode, the developed PAP assay owns a dynamic linear range of 10.0-400 μM with a detection limit of 2.78 μM and provides good practical application performance with above 96.7 ± 4.83% recovery in environmental water and soil samples. Therefore, the PGM-based amplifiable sensor for PAP proposed can accommodate these requirements of environment monitoring and has promising potential for evaluating pollutants in real environmental samples.
提出了一种单靶多触发信号模型传感策略，通过使用商用个人血糖仪（PGM）进行信号读出，以核壳“负载型”纳米材料MSNs@MnO作为可放大纳米探针，快速、灵敏地现场检测环境污染物对氨基苯酚（PAP）。在该设计中，包裹有信号分子葡萄糖的介孔二氧化硅纳米颗粒（MSNs）纳米容器被可氧化还原的二氧化锰（MnO）纳米片包覆，形成可放大纳米探针（Glu-MSNs@MnO）。当遇到PAP时，MnO与PAP之间的氧化还原反应可诱导MSNs@MnO外层降解，释放出多个负载的葡萄糖拷贝，从而点亮PGM信号。由于纳米载体的高负载能力，目标物与信号分子葡萄糖之间存在“一对多”关系，能够产生足够的信号输出，以满足现场测定环境污染物的要求。利用这种放大模式，所开发的PAP检测方法的动态线性范围为10.0 - 400 μM，检测限为2.78 μM，在环境水和土壤样品中的回收率高于96.7±4.83%，具有良好的实际应用性能。因此，所提出的基于PGM的PAP可放大传感器能够满足环境监测的这些要求，在评估实际环境样品中的污染物方面具有广阔的应用前景。
