Departamento de Química, Grupo GEANA, Instituto para el Desarrollo Agroindustrial y de la Salud (IDAS), Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Agencia Postal N(o) 3, 5800, Río Cuarto, Argentina.
Talanta. 2020 Apr 1;210:120647. doi: 10.1016/j.talanta.2019.120647. Epub 2019 Dec 16.
A GCE/CRGO-βCD's/ADA-SPE/AuNPs biosensor was successfully developed to determine eugenol in dental samples. The optimal conditions to construct the biosensor were obtained from an experimental design based on the response surfaces methodology. The GCE/CRGO-βCD/ADA-SPE/AuNPs biosensor exhibited a very good analytical performance for the quantification of eugenol. Thus, it shows a linear range between 1.3 × 10 and 1 × 10 mol L, with a sensitivity of (5.3 ± 0.3) x 10 A mol L. The limits of detection and quantification were 4 × 10 mol L and 1.3 × 10 mol L, respectively. Biosensors had an intraday and inter day reproducibility of 5% and 8%, respectively. The repeatability was of 3%, and the stability was 21 days (a decrease of 30% in current responses was observed after the fourth week). Recovery studies were performed in order to validate the proposed method. Recovery percentages were between 94 and 108%. A value of the apparent Michaellis-Menten constant, K, of 3.1 × 10 mol L was obtained using both Lineweaver-Burk and Eadi-Hofstee methods.
成功研制了一种 GCE/CRGO-βCD/ADA-SPE/AuNPs 生物传感器，用于测定牙样本中的丁香酚。通过基于响应面法的实验设计，获得了构建生物传感器的最佳条件。GCE/CRGO-βCD/ADA-SPE/AuNPs 生物传感器对丁香酚的定量分析具有很好的分析性能。因此，它在 1.3×10 和 1×10 mol L 之间表现出线性范围，灵敏度为（5.3±0.3）x 10 A mol L。检测限和定量限分别为 4×10 mol L 和 1.3×10 mol L。生物传感器的日内和日间重现性分别为 5%和 8%。重复性为 3%，稳定性为 21 天（第四周后电流响应下降 30%）。为了验证所提出的方法，进行了回收研究。回收率在 94%至 108%之间。使用 Lineweaver-Burk 和 Eadi-Hofstee 方法，获得了表观米氏常数 K 的值为 3.1×10 mol L。
