Mary Mathew K, Reshma Ranjanan, Geethu M, Rithin Varghese, Swapna Sasidharan, Gouthaman P P, Sabu K K, Nadiya F, Noushad Muhammad Ali, Dharan Soumya S, Prakashkumar R, Remashree A B
Indian Cardamom Research Institute, Spices Board Min Commerce & Industry, Government of India, Myladumpara, Idukki, Kerala 685553, India.
Jawaharlal Nehru Tropical Botanic Garden and Research Institute, Palode, Thiruvananthapuram 695562, India.
Data Brief. 2020 Jan 7;29:105047. doi: 10.1016/j.dib.2019.105047. eCollection 2020 Apr.
Large cardamom ( Roxburg), is an ancient spice native to North-Eastern India and Southeast Asia, which belongs to the family under the order Large cardamom is mostly affected by a viral disease termed Chirke caused by Large Cardamom Chirke Virus (LCCV). These disease has spread due to drastic changes in the ecosystem, inadequate rain in dry months and absence of good agricultural practices by the farmers resulting in aphid infestations. In the present study, using HiSeq™ 2000 RNA sequencing technology transcriptome sequencing was performed for both control (disease not expressed) and diseased large cardamom leaf tissues. RNA-seq generated 77260968 (7.72 GB) and 72239708 (7.22 GB) paired raw reads for large cardamom control and diseased samples respectively. The raw data were submitted to the NCBI SRA database under the accession numbers SRX2529373 and SRX2529372 and the assembled transcriptomes were submitted to TSA under the accession numbers GIAV01000000 and GIAW01000000 for the control and diseased samples respectively. The raw reads were quality trimmed and assembled using TRINITY assembler which created 156822 (control) and 148953 (diseased) contigs with N50 values 2107 (control) and 2182 (diseased). The data were used to identify the significantly differentially expressed genes between control and diseased samples.
大高良姜(罗克斯伯里)是一种原产于印度东北部和东南亚的古老香料,属于姜目下的科。大高良姜主要受到一种名为奇尔克病的病毒病影响,该病由大高良姜奇尔克病毒(LCCV)引起。由于生态系统的剧烈变化、旱季降雨不足以及农民缺乏良好的农业 practices 导致蚜虫滋生,这种疾病得以传播。在本研究中,使用HiSeq™ 2000 RNA测序技术对对照(未表现疾病)和患病的大高良姜叶片组织进行了转录组测序。RNA-seq分别为大高良姜对照和患病样本生成了77260968(7.72GB)和72239708(7.22GB)的配对原始 reads。原始数据已提交至NCBI SRA数据库,登录号分别为SRX2529373和SRX2529372,组装后的转录组分别以登录号GIAV01000000和GIAW01000000提交至TSA,用于对照和患病样本。对原始 reads 进行了质量修剪,并使用TRINITY组装器进行组装,创建了156822(对照)和148953(患病)个重叠群,N50值分别为2107(对照)和2182(患病)。这些数据用于鉴定对照和患病样本之间显著差异表达的基因。