Mary Mathew K, Reshma Renjanan, Geethu M, Rithin Varghese, Sabu K K, Nadiya F, Noushad Muhammad Ali, Dharan Soumya S, Rao Y S, Remashree A B
Indian Cardamom Research Institute (Spices Board Min Commerce & Industry, Government of India), Myladumpara, Idukki, Kerala 685553, India.
Jawaharlal Nehru Tropical Botanic Garden and Research Institute, Palode, Thiruvananthapuram 695562, India.
Data Brief. 2019 Oct 8;27:104625. doi: 10.1016/j.dib.2019.104625. eCollection 2019 Dec.
Small cardamom ( (L.) Maton, also known as the '' is a rhizomatous herbaceous monocot from the family Zingiberaceae. In the present study, using HiSeq™ 2000 RNA sequencing technology, transcriptome sequencing was performed for both control and disease stressed small cardamom leaf tissues. RNA-seq generated 46,931,637 (101 base) and 31,682,496 (101 base) raw reads and totally 9.93GB and 6.63GB of sequence data for cardamom control and stressed samples respectively. The raw data were submitted to NCBI SRA database of under the accession numbers SRX2512359 and SRX2512358 for the control and diseased samples respectively. The raw reads were quality filtered and assembled using TRINITY assembler which created 1,11,495 (control) and 91,096 (diseased) contigs with N50 values 3013 (control) and 2729 (stressed). The data was further used to identify significantly differentially expressed unigenes between control and stressed samples. Assembled unigenes were further annotated and evaluated to predict the function using publicly available databases and gene annotation tools.
小豆蔻（（L.）马顿，也被称为“”）是姜科的一种具根状茎的草本单子叶植物。在本研究中，使用HiSeq™ 2000 RNA测序技术，对对照和病害胁迫下的小豆蔻叶片组织进行了转录组测序。RNA测序分别为小豆蔻对照和胁迫样本生成了46,931,637（101碱基）和31,682,496（101碱基）条原始读数，以及总计9.93GB和6.63GB的序列数据。原始数据分别以登录号SRX2512359和SRX2512358提交至NCBI SRA数据库，分别对应对照样本和患病样本。对原始读数进行质量过滤，并使用TRINITY组装器进行组装，该组装器创建了111,495个（对照）和91,096个（患病）重叠群，对照样本的N50值为3013，胁迫样本的N50值为2729。这些数据进一步用于鉴定对照和胁迫样本之间显著差异表达的单基因。对组装的单基因进一步进行注释和评估，以使用公开可用的数据库和基因注释工具预测其功能。
