Wile A G, Sparks F C
Cancer. 1977 Feb;39(2):570-4. doi: 10.1002/1097-0142(197702)39:2<570::aid-cncr2820390229>3.0.co;2-f.
Skin was transplanted from male to female C57BL/6JRC mice. Bacillus Calmette-Guérin (BCG) was administered once subcutaneously, intramuscularly, or intraperitoneally either 14 days prior to grafting or on the day of grafting in a dose of 5 X 10(5), 5 X 10(6), or 5 X 10(7) microorganisms. Serum antibody to BCG was assayed using complement fixation. There was a linear inverse relation between skin graft rejection and dose of BCG (p less than .01) such that the lowest dose of BCG was the most effective. The IM and SQ routes were more effective than the IP route. Schedule did not have significant effect on skin graft survival. Time to appearance of antibody correlated directly with dose. The prolonged survival of skin grafts combined with early appearance of antibody to BCG suggested that high doses of BCG could result in antigenic competition.
将雄性C57BL/6JRC小鼠的皮肤移植到雌性小鼠身上。卡介苗（BCG）在移植前14天或移植当天以5×10⁵、5×10⁶或5×10⁷个微生物的剂量进行一次皮下、肌肉或腹腔注射。使用补体结合法检测血清中抗卡介苗抗体。皮肤移植排斥与卡介苗剂量之间存在线性反比关系（p＜0.01），因此最低剂量的卡介苗最为有效。肌肉注射和皮下注射途径比腹腔注射途径更有效。给药方案对皮肤移植存活没有显著影响。抗体出现的时间与剂量直接相关。皮肤移植的延长存活与抗卡介苗抗体的早期出现表明，高剂量的卡介苗可能导致抗原竞争。
