Department of Pharmaceutical Biotechnology, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education, Manipal, Karnataka, 576104, India.
Department of Chemical Engineering, Manipal Institute of Technology, Manipal Academy of Higher Education, Manipal, Karnataka, 576104, India.
Appl Biochem Biotechnol. 2020 May;191(1):135-150. doi: 10.1007/s12010-020-03235-8. Epub 2020 Jan 27.
A bacterial strain PB1 with antagonistic activity against pathogenic fungi was isolated from marine soil and was identified as Paenibacillus elgii based on phenotypic and genotypic characterization. The isolate showed good antifungal activity against "Aspergillus niger (MTCC 282), Trichophyton rubrum (MTCC 791), Microsporum gypseum (MTCC 2819), Candida albicans (MTCC 227), and Saccharomyces cerevisiae (MTCC 170)". Chitinase and beta 1, 4-endoglucanase are known for their capability to degrade fungal cell wall, thus we analyzed its productivity in PB1 strain using Plackett-Burman and Central Composite Design. The factors that affect the productivity of chitinase and beta 1, 4-endoglucanase were identified and optimized. A 7.77-fold increase (3.157 to 24.53 ± 1.33 U/mL) in chitinase and 7.422-fold increase (6.476 to 48.066 ± 0.676 U/mL) in beta 1, 4-endoglucanase versus basal medium was achieved. Chitinase and beta 1, 4-endoglucanase produced by Paenibacillus elgii strain PB1 represents the new source for biotechnological, medical, and agricultural applications.
从海洋土壤中分离到一株具有拮抗真菌活性的细菌菌株 PB1，并通过表型和基因型特征鉴定为解淀粉芽孢杆菌。该分离株对“黑曲霉（MTCC 282）、红色毛癣菌（MTCC 791）、石膏样小孢子菌（MTCC 2819）、白色念珠菌（MTCC 227）和酿酒酵母（MTCC 170）”具有良好的抗真菌活性。几丁质酶和β1,4-内切葡聚糖酶因其能够降解真菌细胞壁而闻名，因此我们使用 Plackett-Burman 和中心复合设计分析了 PB1 菌株的生产力。确定并优化了影响几丁质酶和β1,4-内切葡聚糖酶生产力的因素。与基础培养基相比，几丁质酶的产量提高了 7.77 倍（3.157 至 24.53±1.33 U/mL），β1,4-内切葡聚糖酶的产量提高了 7.422 倍（6.476 至 48.066±0.676 U/mL）。解淀粉芽孢杆菌 PB1 菌株产生的几丁质酶和β1,4-内切葡聚糖酶代表了生物技术、医学和农业应用的新来源。
