National Key Laboratory of Science and Technology on Micro/Nano Fabrication, Department of Micro/Nano Electronics, Shanghai Jiao Tong University, Shanghai, 200240, China.
Institute of Neuroscience and Key Laboratory of Primate Neurobiology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200031, China; University of Chinese Academy of Sciences, Beijing, 100080, China.
Biosens Bioelectron. 2020 Apr 1;153:112009. doi: 10.1016/j.bios.2020.112009. Epub 2020 Jan 9.
Optogenetic-based neuromodulation tools is evolving for the basic neuroscience research in animals combining optical manipulation and electrophysiological recordings. However, current opto-electric integrated devices attaching on cerebral cortex for electrocorticogram (ECoG) still exist potential damage risks for both brain tissue and electrode, due to the mechanical mismatch and brain deformation. Here, we propose a stretchable opto-electric integrated neural interface by integrating serpentine-shaped electrodes and multisite micro-LEDs onto a hyperelastic substrate, as well as a serpentine-shaped metal shielding embedded in recording electrode for low-noise signal acquisition. The delicate structure design, ultrasoft encapsulation and independent fabrication followed by assembly are beneficial to the conformality, reliability and yield. In vitro accelerated deterioration and reciprocating tensile have demonstrated good performance and high stability. In vivo optogenetic activation of focal cortical areas of awaked mouse expressing Channelrhodopsin-2 is realized with simultaneous high-quality recording. We highlight the potential use of this multifunctional neural interface for neural applications.
基于光遗传学的神经调节工具正在发展，用于动物的基础神经科学研究，结合了光学操作和电生理记录。然而，目前用于脑电皮层电图（ECoG）的光电集成设备仍然存在潜在的脑组织和电极损伤风险，这是由于机械失配和大脑变形。在这里，我们通过将蛇形电极和多位点微 LED 集成到超弹性基底上，并在记录电极中嵌入蛇形金属屏蔽层，提出了一种可拉伸的光电集成神经接口，用于低噪声信号采集。精细的结构设计、超柔软的封装和独立的制造工艺，有利于贴合度、可靠性和成品率。体外加速恶化和往复拉伸测试表明，该接口具有良好的性能和高稳定性。通过对表达通道蛋白的清醒小鼠的焦点皮层区域进行光遗传学激活，实现了同时进行高质量记录。我们强调了这种多功能神经接口在神经应用中的潜在用途。
