Nanobioengineering Group, Institute for Bioengineering of Catalonia (IBEC) Barcelona Institute of Science and Technology (BIST), 12 Baldiri Reixac 15-21, Barcelona, 08028, Spain.
Fetal Medicine Research Center, BCNatal. Hospital Clínic and Hospital Sant Joan de Déu, Universitat de Barcelona. Building Helios 2, Sabino Arana Street 1, 08028, Barcelona, Spain.
Biosens Bioelectron. 2020 Apr 1;153:112028. doi: 10.1016/j.bios.2020.112028. Epub 2020 Jan 21.
Oxygen is vital for energy metabolism in mammals and the variability of the concentration is considered a clinical alert for a wide range of metabolic malfunctions in medicine. In this article, we describe the development and application of a micro-needle implantable platinum-based electrochemical sensor for measuring partial pressure of oxygen in intramuscular tissue (in-vivo) and vascular blood (ex-vivo). The Pt-Nafion® sensor was characterized morphological and electrochemically showing a higher sensitivity of -2.496 nA/mmHg (-1.495 nA/μM) when comparing with its bare counterpart. Our sensor was able to discriminate states with different oxygen partial pressures (pO) for ex-vivo (blood) following the same trend of the commercial gas analyzer used as standard. For in-vivo (intramuscular) experiments, since there is not a gold standard for measuring pO in tissue, it was not possible to correlate the obtained currents with the pO in tissue. However, our sensor was able to detect clear statistical differences of O between hyperoxia and hypoxia states in tissue.
氧气对于哺乳动物的能量代谢至关重要，其浓度的变化被认为是医学中广泛的代谢功能障碍的临床警报。在本文中，我们描述了一种基于铂的微针植入式电化学传感器的开发和应用，用于测量肌肉组织（体内）和血管血液（体外）中的氧分压。Pt-Nafion®传感器在形态和电化学方面都得到了表征，与裸 Pt 相比，其灵敏度更高，为-2.496 nA/mmHg（-1.495 nA/μM）。我们的传感器能够区分不同氧分压（pO）的体外（血液）状态，与用作标准的商业气体分析仪呈现相同的趋势。对于体内（肌肉组织）实验，由于目前没有测量组织中 pO 的金标准，因此无法将获得的电流与组织中的 pO 相关联。然而，我们的传感器能够检测到组织中氧在高氧和低氧状态之间的明显统计学差异。
