Department of Epidemiology and Health Statistics, School of Public Health, Guangxi Medical University, Nanning, China.
Department of Epidemiology and Health Statistics, School of Public Health, Guilin Medical University, Guilin, China.
DNA Cell Biol. 2020 Apr;39(4):683-689. doi: 10.1089/dna.2019.5237. Epub 2020 Jan 28.
This study aimed to investigate the expression and clinical significance of chemokine-like factor-like MARVEL transmembrane domain-containing family member 2 (CMTM2) in hepatocellular carcinoma (HCC) tissues. Bioinformatics analysis showed that CMTM2 was downregulated in HCC tissues and correlated with vascular invasion of HCC patients. The immunohistochemistry (IHC) method found that CMTM2 expression was negative in 29/75 (38.67%) cases of HCC tissues and 13/75 (17.33%) cases of paracancerous tissues, also showed a significantly lower expression of CMTM2 in HCC tissues than the paired paracancerous tissues ( < 0.05). Moreover, CMTM2 expression was significantly correlated with tumor grade of HCC patients ( < 0.05), but had no relationship with other clinicopathological features. In addition, multivariate logistic regression analysis indicated that tumor grade was an independent risk factor for CMTM2 expression. The survival time of HCC patients between high and low expression of CMTM2 had no difference by bioinformatics analysis, but the IHC result showed that the negative expression of CMTM2 was related to a poor prognosis of HCC patients. Further COX regression analysis showed that CMTM2 expression was an independent protective factor for the prognosis of HCC patients. We identify that CMTM2 is downregulated in HCC tissues and correlated with the prognosis of HCC patients, suggesting a potential tumor suppressor role of CMTM2 in HCC progression.
本研究旨在探讨趋化因子样因子样 MARVEL 跨膜结构域家族成员 2（CMTM2）在肝细胞癌（HCC）组织中的表达及其临床意义。生物信息学分析表明，CMTM2 在 HCC 组织中下调，并与 HCC 患者的血管侵犯相关。免疫组织化学（IHC）方法发现，在 75 例 HCC 组织中，CMTM2 表达阴性的有 29 例（38.67%），在 75 例癌旁组织中，CMTM2 表达阴性的有 13 例（17.33%），HCC 组织中 CMTM2 的表达明显低于配对的癌旁组织（ < 0.05）。此外，CMTM2 的表达与 HCC 患者的肿瘤分级显著相关（ < 0.05），但与其他临床病理特征无关。此外，多因素 logistic 回归分析表明，肿瘤分级是 CMTM2 表达的独立危险因素。生物信息学分析显示，CMTM2 高表达和低表达的 HCC 患者的生存时间无差异，但 IHC 结果显示 CMTM2 阴性表达与 HCC 患者的预后不良相关。进一步的 COX 回归分析表明，CMTM2 表达是 HCC 患者预后的独立保护因素。我们发现 CMTM2 在 HCC 组织中下调，与 HCC 患者的预后相关，提示 CMTM2 在 HCC 进展中可能具有肿瘤抑制作用。
