Givol D, Zakut R, Effron K, Rechavi G, Ram D, Cohen J B
Nature. 1981 Jul 30;292(5822):426-30. doi: 10.1038/292426a0.
The sequences of four embryonic mouse immunoglobulin VH genes have been compared. All genes end at codon 98 and code for a hydrophobic signal peptide of 19 residues interrupted at codon -4 by an intron of 83 base pairs. Substitutions occur in all gene segments but at a significantly higher frequency in the hypervariable regions. The data suggest an evolutionary basis for the diversity of immunoglobulin genes. Divergence resulted also in a termination codon in two of the genes, suggesting that part of the V gene repertoire cannot be expressed unless some correction mechanism is available.
已对四个胚胎小鼠免疫球蛋白VH基因的序列进行了比较。所有基因均在密码子98处终止，编码一个由19个残基组成的疏水信号肽，该信号肽在密码子-4处被一个83个碱基对的内含子中断。所有基因片段均有替换发生，但在高变区的替换频率明显更高。这些数据表明免疫球蛋白基因多样性存在进化基础。分化还导致其中两个基因出现终止密码子，这表明除非有某种校正机制，否则部分V基因库无法表达。
