不同亚组的免疫球蛋白基因在VK基因座内相互交错。
Immunoglobulin genes of different subgroups are interdigitated within the VK locus.
作者信息
Pech M, Zachau H G
出版信息
Nucleic Acids Res. 1984 Dec 21;12(24):9229-36. doi: 10.1093/nar/12.24.9229.
Abstract
The variable regions of immunoglobulins are encoded by multigene families which are rearranged during B-cell differentiation. These families were classified in groups and subgroups based on their amino acid sequences. Genes belonging to a distinct subgroup are believed to occur in the genome within clusters. We are investigating the organization of human variable region genes of the kappa type (VK genes, ref. 1) in the germline and found now for the first time that VK sequences of three of the four different subgroups are interdigitated within the VK locus. We present evidence for the interspersion of two VKIII genes and a VKII pseudogene within an array of five VKI genes. All eight VK sequences are arranged in the same orientation. An evolutionary model for the generation of this 'mixed cluster' is discussed.
摘要
免疫球蛋白的可变区由多基因家族编码，这些基因家族在B细胞分化过程中发生重排。根据氨基酸序列，这些家族被分为不同的组和亚组。属于不同亚组的基因被认为在基因组中以簇的形式出现。我们正在研究种系中人类κ型可变区基因（VK基因，参考文献1）的组织情况，现在首次发现四个不同亚组中的三个亚组的VK序列在VK基因座内相互交错。我们提供了证据，证明两个VKIII基因和一个VKII假基因散布在五个VKI基因的阵列中。所有八个VK序列都以相同的方向排列。本文讨论了这种“混合簇”产生的进化模型。
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