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1
Isolation of a complementary DNA clone encoding a precursor to human eosinophil major basic protein.编码人类嗜酸性粒细胞主要碱性蛋白前体的互补DNA克隆的分离
J Exp Med. 1988 Dec 1;168(6):2295-308. doi: 10.1084/jem.168.6.2295.
2
Acidic precursor revealed in human eosinophil granule major basic protein cDNA.人类嗜酸性粒细胞颗粒主要碱性蛋白cDNA中揭示的酸性前体。
J Exp Med. 1988 Oct 1;168(4):1493-8. doi: 10.1084/jem.168.4.1493.
3
Human eosinophil major basic protein, a mediator of allergic inflammation, is expressed by alternative splicing from two promoters.人类嗜酸性粒细胞主要碱性蛋白是一种过敏性炎症介质，由两个启动子通过可变剪接表达。
Biochem J. 1995 Feb 1;305 ( Pt 3)(Pt 3):921-7. doi: 10.1042/bj3050921.
4
Regulation and processing of a precursor form of eosinophil granule major basic protein (ProMBP) in differentiating eosinophils.分化中的嗜酸性粒细胞中嗜酸性粒细胞颗粒主要碱性蛋白前体形式（ProMBP）的调控与加工
Blood. 1998 Jul 15;92(2):623-31.
5
Cloning and sequence analysis of the human gene encoding eosinophil major basic protein.
Gene. 1990 Feb 14;86(2):285-9. doi: 10.1016/0378-1119(90)90292-y.
6
The identification and cloning of a murine major basic protein gene expressed in eosinophils.
J Immunol. 1995 Sep 15;155(6):3002-12.
7
Sequencing and cloning of the cDNA of guinea pig eosinophil major basic protein.豚鼠嗜酸性粒细胞主要碱性蛋白cDNA的测序与克隆
FEBS Lett. 1991 Feb 25;279(2):330-4. doi: 10.1016/0014-5793(91)80181-2.
8
Cloning of cDNA for rat eosinophil major basic protein.大鼠嗜酸性粒细胞主要碱性蛋白cDNA的克隆
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Eosinophil cationic protein cDNA. Comparison with other toxic cationic proteins and ribonucleases.嗜酸性粒细胞阳离子蛋白互补脱氧核糖核酸。与其他毒性阳离子蛋白和核糖核酸酶的比较。
J Immunol. 1989 Aug 1;143(3):952-5.
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Human eosinophil cationic protein. Molecular cloning of a cytotoxin and helminthotoxin with ribonuclease activity.人嗜酸性粒细胞阳离子蛋白。一种具有核糖核酸酶活性的细胞毒素和抗蠕虫毒素的分子克隆。
J Exp Med. 1989 Jul 1;170(1):163-76. doi: 10.1084/jem.170.1.163.

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Hum Mol Genet. 2023 Feb 19;32(5):790-797. doi: 10.1093/hmg/ddac238.
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Application of Proteomics to Inflammatory Bowel Disease Research: Current Status and Future Perspectives.蛋白质组学在炎症性肠病研究中的应用：现状与未来展望
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Using guinea pigs in studies relevant to asthma and COPD.在与哮喘和慢性阻塞性肺疾病相关的研究中使用豚鼠。
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Human eosinophil major basic protein, a mediator of allergic inflammation, is expressed by alternative splicing from two promoters.人类嗜酸性粒细胞主要碱性蛋白是一种过敏性炎症介质，由两个启动子通过可变剪接表达。
Biochem J. 1995 Feb 1;305 ( Pt 3)(Pt 3):921-7. doi: 10.1042/bj3050921.
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Molecular cloning of the human eosinophil-derived neurotoxin: a member of the ribonuclease gene family.人嗜酸性粒细胞衍生神经毒素的分子克隆：核糖核酸酶基因家族的一个成员。
Proc Natl Acad Sci U S A. 1989 Jun;86(12):4460-4. doi: 10.1073/pnas.86.12.4460.
7
Antibiotic proteins of human polymorphonuclear leukocytes.人类多形核白细胞的抗生素蛋白。
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8
Human eosinophil cationic protein. Molecular cloning of a cytotoxin and helminthotoxin with ribonuclease activity.人嗜酸性粒细胞阳离子蛋白。一种具有核糖核酸酶活性的细胞毒素和抗蠕虫毒素的分子克隆。
J Exp Med. 1989 Jul 1;170(1):163-76. doi: 10.1084/jem.170.1.163.
9
Azurocidin and a homologous serine protease from neutrophils. Differential antimicrobial and proteolytic properties.天青杀素与中性粒细胞中的一种同源丝氨酸蛋白酶。抗菌和蛋白水解特性的差异
J Clin Invest. 1990 Mar;85(3):904-15. doi: 10.1172/JCI114518.
10
Bactenecins, defense polypeptides of bovine neutrophils, are generated from precursor molecules stored in the large granules.杆菌防御素是牛中性粒细胞的防御性多肽，由储存在大颗粒中的前体分子产生。
J Cell Biol. 1990 Oct;111(4):1363-71. doi: 10.1083/jcb.111.4.1363.

本文引用的文献

1
Possible role of flanking nucleotides in recognition of the AUG initiator codon by eukaryotic ribosomes.侧翼核苷酸在真核生物核糖体识别AUG起始密码子中的可能作用。
Nucleic Acids Res. 1981 Oct 24;9(20):5233-52. doi: 10.1093/nar/9.20.5233.
2
Elevated levels of the eosinophil granule major basic protein in the sputum of patients with bronchial asthma.支气管哮喘患者痰液中嗜酸性粒细胞颗粒主要碱性蛋白水平升高。
Mayo Clin Proc. 1981 Jun;56(6):345-53.
3
A simple method for displaying the hydropathic character of a protein.一种展示蛋白质亲水性特征的简单方法。
J Mol Biol. 1982 May 5;157(1):105-32. doi: 10.1016/0022-2836(82)90515-0.
4
An immunofluorescent method for specific staining of eosinophil granule major basic protein.一种用于嗜酸性粒细胞颗粒主要碱性蛋白特异性染色的免疫荧光方法。
J Immunol Methods. 1981;47(2):227-38. doi: 10.1016/0022-1759(81)90123-x.
5
Destruction of bloodstream forms of Trypanosoma cruzi by eosinophil granule major basic protein.嗜酸性粒细胞颗粒主要碱性蛋白对克氏锥虫血流形式的破坏作用
Am J Trop Med Hyg. 1981 Jul;30(4):775-9. doi: 10.4269/ajtmh.1981.30.775.
6
Organization and expression of eucaryotic split genes coding for proteins.编码蛋白质的真核生物断裂基因的组织与表达。
Annu Rev Biochem. 1981;50:349-83. doi: 10.1146/annurev.bi.50.070181.002025.
7
Distinctive cationic proteins of the human eosinophil granule: major basic protein, eosinophil cationic protein, and eosinophil-derived neurotoxin.人类嗜酸性粒细胞颗粒的特异性阳离子蛋白：主要碱性蛋白、嗜酸性粒细胞阳离子蛋白和嗜酸性粒细胞衍生神经毒素。
J Immunol. 1983 Dec;131(6):2977-82.
8
Peptide antibiotic subtilin is synthesized via precursor proteins.肽类抗生素枯草菌素是通过前体蛋白合成的。
Biochem Biophys Res Commun. 1983 Oct 31;116(2):751-8. doi: 10.1016/0006-291x(83)90588-0.
9
Localization of a molecule immunochemically similar to eosinophil major basic protein in human placenta.人胎盘中一种免疫化学性质类似于嗜酸性粒细胞主要碱性蛋白的分子的定位。
J Exp Med. 1984 Jul 1;160(1):29-41. doi: 10.1084/jem.160.1.29.
10
Cationic antimicrobial proteins isolated from human neutrophil granulocytes in the presence of diisopropyl fluorophosphate.在二异丙基氟磷酸存在的情况下从人中性粒细胞中分离出的阳离子抗菌蛋白。
Infect Immun. 1984 Jul;45(1):29-35. doi: 10.1128/iai.45.1.29-35.1984.

编码人类嗜酸性粒细胞主要碱性蛋白前体的互补DNA克隆的分离

Isolation of a complementary DNA clone encoding a precursor to human eosinophil major basic protein.

作者信息

McGrogan M, Simonsen C, Scott R, Griffith J, Ellis N, Kennedy J, Campanelli D, Nathan C, Gabay J

机构信息

Invitron Corporation, Redwood City, California 94063.

出版信息

J Exp Med. 1988 Dec 1;168(6):2295-308. doi: 10.1084/jem.168.6.2295.

DOI:10.1084/jem.168.6.2295
PMID:3199069
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2189145/
Abstract

A 14-kD protein was purified from human PMNs and its NH2-terminal sequence was determined. Comparison of a portion of the NH2-terminal sequence of this protein to the recently reported NH2-terminal sequence of eosinophil major basic protein (MBP) showed them to be identical. To aid further characterization of the structural and functional properties of this molecule, we isolated from an HL-60 cDNA library a single class of cDNA clones whose sequence matched exactly the NH2-terminal amino acid sequence of the 14-kD polypeptide. Northern analysis of HL-60 cells suggests that MBP is constitutively expressed in HL-60 cells and is highly transcribed from a single copy gene. The sequence of the full-length cDNA clones predicts that MBP is synthesized as a 23-kD precursor form (pro-MBP) which is subsequently cleaved to release the mature 14-kD MBP. The putative pro-MBP has a predicted pI of 6.0, but both the charged and the hydrophobic residues are asymmetrically distributed, creating a bipolar molecule. The NH2-terminal half has a predicted pI of 3.7 and is hydrophilic, while the COOH-terminal half (corresponding to mature MBP) has a predicted pI of 11.1 and is hydrophobic.

摘要

从人多形核白细胞中纯化出一种14-kD蛋白，并测定了其氨基末端序列。将该蛋白的部分氨基末端序列与最近报道的嗜酸性粒细胞主要碱性蛋白（MBP）的氨基末端序列进行比较，发现它们是相同的。为了进一步表征该分子的结构和功能特性，我们从HL-60 cDNA文库中分离出一类cDNA克隆，其序列与14-kD多肽的氨基末端氨基酸序列完全匹配。对HL-60细胞的Northern分析表明，MBP在HL-60细胞中组成性表达，并且从单拷贝基因高度转录。全长cDNA克隆的序列预测，MBP以23-kD前体形式（前MBP）合成，随后被切割以释放成熟的14-kD MBP。推测的前MBP的预测等电点为6.0，但带电和疏水残基均不对称分布，形成双极分子。氨基末端一半的预测等电点为3.7，是亲水性的，而羧基末端一半（对应于成熟MBP）的预测等电点为11.1，是疏水性的。