Nishio C, Komura S, Kurahashi K
Biochem Biophys Res Commun. 1983 Oct 31;116(2):751-8. doi: 10.1016/0006-291x(83)90588-0.
Biogenesis of subtilin, an antimicrobial peptide produced by Bacillus subtilis ATCC 6633, was studied in growing cells. Pulse-chase labeling experiments with [35S]cysteine revealed the presence of precursor proteins of subtilin. The synthesis of both precursor proteins and subtilin was inhibited by inhibitors of protein and RNA synthesis. When the precursor proteins were incubated with crude extracts of the organism in vitro, they were converted to subtilin. Pepstatin and phenylmethylsulfonyl fluoride in combination inhibited this conversion.
对枯草芽孢杆菌ATCC 6633产生的抗菌肽枯草菌素在生长细胞中的生物合成进行了研究。用[35S]半胱氨酸进行脉冲追踪标记实验揭示了枯草菌素前体蛋白的存在。前体蛋白和枯草菌素的合成均受到蛋白质和RNA合成抑制剂的抑制。当将前体蛋白与该生物体的粗提物在体外孵育时，它们会转化为枯草菌素。胃蛋白酶抑制剂和苯甲基磺酰氟联合使用可抑制这种转化。
