Kennedy H E, Jones B V, Tucker E M, Ford N J, Clarke S W, Furze J, Thomas L H, Stott E J
Institute for Animal Health, Compton Laboratory, Newbury, Berkshire, U.K.
J Gen Virol. 1988 Dec;69 ( Pt 12):3023-32. doi: 10.1099/0022-1317-69-12-3023.
Six interspecific hybridomas (heterohybridomas) secreting bovine monoclonal antibodies (MAbs) against respiratory syncytial (RS) virus were produced. Four of the heterohybridomas were formed using the mouse myeloma cell line NS1 as the fusion partner, one using NS0, and the remaining heterohybridoma was formed using a bovine X murine hybridoma as the fusion partner. Five heterohybridomas secreted bovine IgG1 and one secreted IgG2. All six MAbs recognized human subtype A and B viruses as well as bovine RS virus. They were specific for the fusion glycoprotein and reacted with a 140K dimer and a 70K monomer in a Western blot of native antigen; three also bound to the 46K F1 component and its 22K cleavage product in a blot of reduced antigen. Two of these MAbs neutralized RS virus infectivity, inhibited virus-induced fusion, lysed RS virus-infected cells in the presence of complement and protected mice against RS virus challenge.
制备了六种分泌抗呼吸道合胞（RS）病毒牛单克隆抗体（MAb）的种间杂交瘤（异种杂交瘤）。其中四种异种杂交瘤是用小鼠骨髓瘤细胞系NS1作为融合伙伴形成的，一种用NS0，其余异种杂交瘤是用牛X小鼠杂交瘤作为融合伙伴形成的。五种异种杂交瘤分泌牛IgG1，一种分泌IgG2。所有六种单克隆抗体都能识别人类A和B亚型病毒以及牛RS病毒。它们对融合糖蛋白具有特异性，并在天然抗原的蛋白质印迹中与140K二聚体和70K单体发生反应；三种单克隆抗体在还原抗原的印迹中也与46K F1成分及其22K裂解产物结合。其中两种单克隆抗体可中和RS病毒感染性，抑制病毒诱导的融合，在补体存在下裂解RS病毒感染的细胞，并保护小鼠免受RS病毒攻击。
