College of Veterinary Medicine & Institute of Animal Medicine, Gyeongsang National University, Jinju, 52828, Republic of Korea.
Department of Veterinary Paraclinical Sciences, College of Veterinary Medicine, University of the Philippines Los Banos, College, Laguna, 4031, Philippines.
Dev Comp Immunol. 2020 May;106:103633. doi: 10.1016/j.dci.2020.103633. Epub 2020 Jan 25.
Riemerella anatipestifer causes infectious disease and considerable economic loss in the duck industry worldwide. Our previous studies demonstrated an association between proinflammatory cytokine interleukin (IL)-17A and R. anatipestifer infection. Here, we provide evidence for IL-17A involvement in R. anatipestifer infection using a mouse model. Mice showed higher resistance to R. anatipestifer infection than ducks, with median lethal doses (LD) of 3.5 × 10 and 5 × 10 colony-forming units (CFU), respectively. Twenty-four hours after infection, mice with a sub-lethal dose (3.5 × 10 CFU) exhibited levels of IL-17A and IL-23 expression similar to uninfected mice. Thus, we hypothesized that exogenous IL-17A or IL-23 administration affects susceptibility of mice to R. anatipestifer. Mice pretreated with IL-17A or IL-23 prior to sub-lethal dose infection of R. anatipestifer exhibited increased bacterial burden and spleen weights compared to untreated infected mice, confirming the involvement of IL-17A in susceptibility to R. anatipestifer infection in vivo.
鸭疫里默氏杆菌引起的传染病给全球养鸭业造成了巨大的经济损失。我们之前的研究表明，促炎细胞因子白细胞介素（IL）-17A 与鸭疫里默氏杆菌感染之间存在关联。在这里，我们使用小鼠模型为 IL-17A 参与鸭疫里默氏杆菌感染提供了证据。与鸭相比，小鼠对鸭疫里默氏杆菌感染的抵抗力更高，其半数致死剂量（LD）分别为 3.5×10 和 5×10 菌落形成单位（CFU）。感染后 24 小时，接受亚致死剂量（3.5×10 CFU）感染的小鼠的 IL-17A 和 IL-23 表达水平与未感染的小鼠相似。因此，我们假设外源性 IL-17A 或 IL-23 给药会影响小鼠对鸭疫里默氏杆菌的易感性。与未经处理的感染小鼠相比，在亚致死剂量的鸭疫里默氏杆菌感染前用 IL-17A 或 IL-23 预处理的小鼠的细菌负荷和脾脏重量增加，证实了 IL-17A 参与了体内对鸭疫里默氏杆菌感染的易感性。
