Key Laboratory of Hunan Province for Water Environment and Agriculture Product Safety, College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, PR China.
Department of Clinical Pharmacology, Xiangya Hospital, Hunan Key Laboratory of Pharmacogenetics, Central South University, 410078 Changsha, PR China.
Food Chem. 2020 Jun 15;315:126171. doi: 10.1016/j.foodchem.2020.126171. Epub 2020 Jan 9.
A new-style white pepper derived dual-emission carbon dots (CDs) with a quantum yield of 10.4% was designed and facile constructed with one-pot solvothermal method. The green emission (520 nm) had an efficient and special "turn-on" fluorescence sensing of coenzyme A (CoA) with the aid of Cu, while red emission (668 nm) barely changed and worked as reference. In the concentration range (0-150 µM), relative fluorescence intensity ratios (F/F) showed excellent linear correlation with concentrations of CoA, and detection limit was as low as 8.75 nm. Moreover, the strategy has been successfully applied for label-free detection of CoA in real pig liver samples with good recoveries (93.3-108.0%). Notably, the synthesized CDs had durable fluorescence, low cytotoxicity, and good biocompatibility for cellular imaging, which demonstrated wide and promising applicability for biosensing and bioimaging in the future.
一种新型的白色胡椒衍生的双发射碳点（CDs），量子产率为 10.4%，采用一锅溶剂热法设计并简便地构建而成。绿色发射（520nm）在 Cu 的辅助下对辅酶 A（CoA）具有高效且特殊的“开启”荧光传感，而红色发射（668nm）几乎不变，用作参考。在浓度范围（0-150µM）内，相对荧光强度比（F/F）与 CoA 的浓度呈良好的线性相关，检测限低至 8.75nm。此外，该策略已成功应用于无标记检测实际猪肝样品中的 CoA，回收率良好（93.3-108.0%）。值得注意的是，所合成的 CDs 具有持久的荧光、低细胞毒性和良好的细胞成像生物相容性，为未来的生物传感和生物成像展示了广泛而有前途的适用性。
