新诊断2型糖尿病患者中性粒细胞弹性蛋白酶和髓过氧化物酶mRNA的表达
Expression of neutrophil elastase and myeloperoxidase mRNA in patients with newly diagnosed type 2 diabetes mellitus.
作者信息
Ferdous Mokerroma, R Sonam C, Mudi Sonchita R, Ali Mohammad, Jasmin Shahana, Fariduddin Mohammad, Alam Sheikh M K, Arslan M I, Biswas Subrata K
机构信息
Department of Biochemistry and Molecular Biology, Bangabandhu Sheikh Mujib Medical University (BSMMU), Dhaka, Bangladesh.
Department of Biochemistry, Kumudini Women's Medical College, Mirzapur, Tangail, Bangladesh.
出版信息
Diabetes Metab Syndr. 2020 Mar-Apr;14(2):83-85. doi: 10.1016/j.dsx.2020.01.001. Epub 2020 Jan 8.
BACKGROUND AND AIMS
Neutrophil elastase and myeloperoxidase enzymes protect us from infection by killing pathogens. However, exaggerated activities of these enzymes can induce tissue damage, inflammation and oxidative stress. The present study was aimed to explore the expressions of neutrophil elastase and myeloperoxidase mRNA in the peripheral blood leukocytes (PBL) in patients with newly diagnosed type 2 diabetes mellitus.
METHODS
In this cross-sectional study, 104 participants including 65 normoglycemic control subjects and 39 newly diagnosed type 2 diabetes patients were recruited. Glycemic and metabolic markers were evaluated from fasting blood samples. The mRNA levels of neutrophil elastase and myeloperoxidase genes in the PBL were quantified by real-time quantitative PCR.
RESULTS
Compared to control subjects, diabetes patients showed a significant down regulation of both neutrophil elastase (p = 0.039) and myeloperoxidase (p = 0.023) mRNA expressions in the PBL. The neutrophil elastase and myeloperoxidase mRNA levels showed a negative trend with fasting glucose levels but did not show any significant correlations with HbA1c, insulin level, insulin resistance or sensitivity status.
CONCLUSIONS
It was concluded that type 2 diabetes mellitus is associated with a decrease in neutrophil elastase and myeloperoxidase gene expression in the PBL.
背景与目的
中性粒细胞弹性蛋白酶和髓过氧化物酶可通过杀灭病原体来保护我们免受感染。然而，这些酶的过度活性会导致组织损伤、炎症和氧化应激。本研究旨在探讨新诊断的2型糖尿病患者外周血白细胞（PBL）中中性粒细胞弹性蛋白酶和髓过氧化物酶mRNA的表达情况。
方法
在这项横断面研究中，招募了104名参与者，包括65名血糖正常的对照者和39名新诊断的2型糖尿病患者。从空腹血样中评估血糖和代谢指标。通过实时定量PCR定量PBL中中性粒细胞弹性蛋白酶和髓过氧化物酶基因的mRNA水平。
结果
与对照者相比，糖尿病患者PBL中中性粒细胞弹性蛋白酶（p = 0.039）和髓过氧化物酶（p = 0.023）的mRNA表达均显著下调。中性粒细胞弹性蛋白酶和髓过氧化物酶的mRNA水平与空腹血糖水平呈负相关趋势，但与糖化血红蛋白、胰岛素水平、胰岛素抵抗或敏感性状态无显著相关性。
结论
得出结论，2型糖尿病与PBL中中性粒细胞弹性蛋白酶和髓过氧化物酶基因表达降低有关。
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