Ali Mohammad, Jasmin Shahana, Fariduddin Mohammad, Alam Sheikh M K, Arslan M I, Biswas Subrata K
Department of Biochemistry and Molecular Biology, Bangabandhu Sheikh Mujib Medical University (BSMMU), Shahbag, Dhaka - 1000, Bangladesh.
Department of Endocrinology, Bangabandhu Sheikh Mujib Medical University (BSMMU), Dhaka, Bangladesh.
Mol Biol Rep. 2018 Oct;45(5):1245-1252. doi: 10.1007/s11033-018-4279-4. Epub 2018 Jul 28.
Neutrophil elastase and myeloperoxidase enzymes have been implicated in high-fat diet-induced obesity, insulin resistance (IR) and atherosclerosis in animal models. The aim of the present study was to explore neutrophil elastase and myeloperoxidase mRNA expressions in the peripheral blood leukocytes (PBL) in overweight and obese subjects, and to correlate those mRNA expressions with BMI, IR and cardiovascular biomarkers. In this cross-sectional study, 74 apparently healthy subjects including 22 lean, 27 overweight and 25 obese subjects were recruited. Cardiovascular and metabolic biomarkers were evaluated from fasting blood samples. The mRNA levels of neutrophil elastase and myeloperoxidase genes in the PBL were quantified by real-time PCR. Compared to lean group, the overweight and obese groups showed significant upregulation of both neutrophil elastase (p < 0.001) and myeloperoxidase (p < 0.03) mRNA expressions in the PBL. But no difference was found between overweight and obese groups. The neutrophil elastase and myeloperoxidase mRNA levels showed significant positive correlation with BMI, serum triglyceride, atherogenic index of plasma and 10-year risk of developing cardiovascular disease. But no correlation was found with glucose, insulin or IR. It was concluded that the neutrophil elastase and myeloperoxidase genes are up-regulated in both overweight and obese subjects and are associated with BMI and markers of cardiovascular disease.
在动物模型中，中性粒细胞弹性蛋白酶和髓过氧化物酶与高脂饮食诱导的肥胖、胰岛素抵抗（IR）和动脉粥样硬化有关。本研究的目的是探讨超重和肥胖受试者外周血白细胞（PBL）中中性粒细胞弹性蛋白酶和髓过氧化物酶的mRNA表达，并将这些mRNA表达与体重指数（BMI）、IR和心血管生物标志物相关联。在这项横断面研究中，招募了74名明显健康的受试者（包括22名瘦人、27名超重者和25名肥胖者）。从空腹血样中评估心血管和代谢生物标志物。通过实时聚合酶链反应（PCR）对PBL中中性粒细胞弹性蛋白酶和髓过氧化物酶基因的mRNA水平进行定量。与瘦人组相比，超重和肥胖组PBL中中性粒细胞弹性蛋白酶（p < 0.001）和髓过氧化物酶（p < 0.03）的mRNA表达均显著上调。但超重和肥胖组之间未发现差异。中性粒细胞弹性蛋白酶和髓过氧化物酶的mRNA水平与BMI、血清甘油三酯、血浆致动脉粥样硬化指数和10年心血管疾病发病风险呈显著正相关。但与血糖、胰岛素或IR无相关性。结论是，超重和肥胖受试者中中性粒细胞弹性蛋白酶和髓过氧化物酶基因均上调，且与BMI和心血管疾病标志物相关。
