Univeristy of Lodz, Department of General Biochemistry, Faculty of Biology and Environmental Protection, 90-236 Łódź, Poland.
State Research Institute, Department of Biochemistry, Institute of Soil Science and Plant Cultivation, 24-100 Puławy, Poland.
Biomed Pharmacother. 2020 Apr;124:109897. doi: 10.1016/j.biopha.2020.109897. Epub 2020 Jan 25.
Sea buckthorn (Elaeagnus rhamnoides (L.) A. Nelson) is a shrub with lanceolate leaves and orange fruits. In traditional Chinese medicine, sea buckthorn organs, especially fruits have been used to treat different diseases, for example cardiovascular disorders. In vitro studies indicate that the phenolic-rich fraction of sea buckthorn fruits demonstrates anti-platelet activity. The aim of the present study was therefore to determine the influence of phenolic and non-polar fractions isolated from the leaves and twigs of sea buckthorn on various parameters of human blood platelets in vitro. Plant material consisted of four different fractions: (1) the phenolic fraction isolated from the leaves, (2) the phenolic fraction isolated from the twigs, (3) the non-polar fraction isolated from leaves and (4) the non-polar fraction from twigs. The chemical composition of the tested fractions was determined using reversed phase UHPLC-HRMS/MS. The fractions from twigs were found to have stronger anti-platelet properties than those from leaves, and all tested fractions were found to be safe for the blood platelets. The tested fractions from the sea buckthorn, especially the non-polar fraction from the twigs, may potentially be a source of compounds with antiplatelet activity.
沙棘（Elaeagnus rhamnoides (L.) A. Nelson）是一种具有披针形叶子和橙色果实的灌木。在中国传统医学中，沙棘器官，特别是果实，已被用于治疗各种疾病，例如心血管疾病。体外研究表明，沙棘果实中的多酚丰富部分具有抗血小板活性。因此，本研究的目的是确定从沙棘叶和嫩枝中分离出的酚类和非极性部分对体外人血小板的各种参数的影响。植物材料由四个不同的部分组成：（1）从叶子中分离出的酚类部分，（2）从嫩枝中分离出的酚类部分，（3）从叶子中分离出的非极性部分，以及（4）从嫩枝中分离出的非极性部分。使用反相 UHPLC-HRMS/MS 测定了测试部分的化学成分。与来自叶子的部分相比，嫩枝部分被发现具有更强的抗血小板特性，并且所有测试部分都被发现对血小板是安全的。来自沙棘的测试部分，特别是来自嫩枝的非极性部分，可能是具有抗血小板活性的化合物的潜在来源。
