Department of Immunology and Infectious Biology, Institute of Microbiology, Biotechnology and Immunology, Faculty of Biology and Environmental Protection, University of Lodz, Banacha 12/16, 90-237 Lodz, Poland.
Department of Biochemistry, Institute of Soil Science and Plant Cultivation, State Research Institute, Czartoryskich 8, 24-100 Pulawy, Poland.
Molecules. 2020 May 9;25(9):2238. doi: 10.3390/molecules25092238.
Biological potential of plant extracts are widely described. Because their oral or topical administration is usually recommended, intestinal mucous and skin are the first surfaces exposed to such preparations. Therefore, we asked the question whether phenolic and non-polar fractions of the extracts from fruits, twigs, and leaves of sea buckthorn ( (L.) A. Nelson) would be able to modulate the functions of human physiological barrier. The study was carried on caucasian colon epithelial-like Caco-2 cells and human foreskin fibroblasts HFF-1 line. Cell secretory activity (ELISA), the expression of cell surface molecules (flow cytometry), cell migration during wound healing in vitro (scratch assay) were assessed. It was demonstrated for the first time, that sea buckthorn extracts can improve intestinal and skin barrier by increasing of ICAM-1 expression on colon epithelial cells and intensification of IL-8 production by fibroblasts. On the other hand, an inhibition of fibroblasts migration in the presence of those preparations was noted. Therefore, greater attention should be paid on precise description of plant extracts effect depended on target cells and their role to give adequate recommendations for such preparations use.
植物提取物的生物学潜力得到了广泛的描述。由于它们通常被推荐口服或局部使用，肠道黏膜和皮肤是首先接触这些制剂的表面。因此，我们提出了这样一个问题：沙棘（Hippophaë rhamnoides L.）的果实、小枝和叶子的提取物中的酚类和非极性部分是否能够调节人体生理屏障的功能。本研究在白种人结肠上皮样 Caco-2 细胞和人包皮成纤维细胞 HFF-1 系上进行。通过细胞分泌活性（ELISA）、细胞表面分子的表达（流式细胞术）和体外伤口愈合过程中的细胞迁移（划痕试验）来评估。这是首次证明，沙棘提取物可以通过增加结肠上皮细胞上 ICAM-1 的表达和增强成纤维细胞产生 IL-8，来改善肠道和皮肤屏障。另一方面，在存在这些制剂的情况下，注意到成纤维细胞迁移受到抑制。因此，应该更加注意根据目标细胞及其作用来精确描述植物提取物的效果，以便为这些制剂的使用提供适当的建议。
