School of Life Sciences, Arizona State University, Tempe, AZ 85287, USA.
The Biodesign Center for Fundamental and Applied Microbiomics, Arizona State University, Tempe, AZ 85287, USA.
Viruses. 2020 Jan 26;12(2):143. doi: 10.3390/v12020143.
The Sonoran Desert tortoise is adapted to the desert, and plays an important ecological role in this environment. There is limited information on the viral diversity associated with tortoises (family Testudinidae), and to date no DNA virus has been identified associated with these animals. This study aimed to assess the diversity of DNA viruses associated with the Sonoran Desert tortoise by sampling their fecal matter. A viral metagenomics approach was used to identify the DNA viruses in fecal samples from wild Sonoran Desert tortoises in Arizona, USA. In total, 156 novel single-stranded DNA viruses were identified from 40 fecal samples. Those belonged to two known viral families, the ( = 27) and ( = 119). In addition, 10 genomes were recovered that belong to the unclassified group of circular-replication associated protein encoding single-stranded (CRESS) DNA virus and five circular molecules encoding viral-like proteins.
索诺兰沙漠龟适应沙漠环境，在这种环境中发挥着重要的生态作用。有关龟类（Testudinidae 科）相关病毒的多样性信息有限，迄今为止，尚未发现与这些动物相关的 DNA 病毒。本研究旨在通过采样粪便来评估与索诺兰沙漠龟相关的 DNA 病毒的多样性。通过使用病毒宏基因组学方法，从美国亚利桑那州的野生索诺兰沙漠龟的粪便样本中鉴定出 DNA 病毒。总共从 40 个粪便样本中鉴定出 156 种新型单链 DNA 病毒。这些病毒属于两个已知的病毒科，Circoviridae（=27）和Parvoviridae（=119）。此外，还回收了 10 个属于未分类的圆形复制相关蛋白编码单链（CRESS）DNA 病毒组和 5 个编码病毒样蛋白的圆形分子。
