Department of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan.
Am J Primatol. 1983;4(1):73-80. doi: 10.1002/ajp.1350040107.
The effect of testosterone on regulation of epididymal protein synthesis has been investigated in castrated rhesus monkeys (Macaca mulatta). The proteins in the treated monkeys were characterized using polyacrylamide gel electrophoresis (under nondenaturing and denaturing conditions) and electrofocusing. At least four distinct proteins have been shown to be synthesized by the monkey epididymis under testosterone influence. Two of these proteins were detected following two days of testosterone treatment while the other two proteins were detected after a six-day treatment period. None of these proteins was detectable in monkeys treated with estradiol for six days. Electrofocusing of epididymal cytosol proteins from untreated and testosterone-treated and castrated monkeys also confirmed the presence of four androgen-dependent proteins in this species. The isoelectric points of these proteins were shown to range between 5.8 and 6.4. The molecular weights of these proteins were found to vary between 47,500 and 66,000. The in vitro incorporation of H-labeled amino acids was markedly greater in the androgen-primed epididymis as compared with the control tissue.
已在去势猕猴（Macaca mulatta）中研究了睾酮对附睾蛋白合成的调节作用。用聚丙烯酰胺凝胶电泳（非变性和变性条件下）和等电聚焦法对处理后的猴子的蛋白质进行了表征。至少有四种不同的蛋白质已被证明在睾酮的影响下由猴附睾合成。其中两种蛋白质在接受两天的睾酮处理后被检测到，而另外两种蛋白质在六天的处理期后被检测到。在接受雌二醇治疗六天的猴子中，未检测到这些蛋白质。未经处理和经睾酮处理及去势的猕猴附睾胞质蛋白的等电聚焦也证实了在该物种中存在四种雄激素依赖性蛋白质。这些蛋白质的等电点显示在 5.8 至 6.4 之间。这些蛋白质的分子量发现介于 47500 和 66000 之间。与对照组织相比，雄激素预处理的附睾中的 H 标记氨基酸的体外掺入明显更高。
