Department of Rehabilitation Sciences, Kobe University Graduate School of Health Sciences, Kobe, Japan.
Faculty of Health Sciences, Kobe University School of Medicine, Kobe, Japan.
Acta Histochem. 2020 Apr;122(3):151511. doi: 10.1016/j.acthis.2020.151511. Epub 2020 Jan 25.
Migration of the macrophages to the injured site soon after the skeletal muscle injury is crucial for subsequent regeneration of the muscle fibers. The Monocyte chemoattractant protein-1 (MCP-1) is important chemokine for regulating migration of the monocytes/macrophages. Earlier reports have discussed that icing applied soon after muscle crush injury retards muscle regeneration through retardation of macrophage migration. The MCP-1+ cells and neutrophils might promote the migration of the macrophages. To test the hypothesis that icing soon after the skeletal muscle injury affects MCP-1+ cells and neutrophils, we examined the effect of icing on MCP-1+ cells and neutrophils after crush injury to skeletal muscle in rats. Owing to the icing application for 20 min soon after the injury, accumulation of the macrophages was inhibited until 12 h after injury. Numbers of the neutrophils at 3 h after the injury and the MCP-1+ cells at 6 h and later after the injury in the icing group were significantly lower than those in the non-icing group, suggesting that these phenomena contribute to the retardation of macrophage migration.
在骨骼肌损伤后不久，巨噬细胞向损伤部位迁移对于随后的肌纤维再生至关重要。单核细胞趋化蛋白-1（MCP-1）是调节单核细胞/巨噬细胞迁移的重要趋化因子。早期的研究报告讨论了冰敷应用于肌肉挤压伤后，通过延迟巨噬细胞迁移来延缓肌肉再生。MCP-1+细胞和中性粒细胞可能促进巨噬细胞的迁移。为了验证骨骼肌损伤后立即冰敷会影响 MCP-1+细胞和中性粒细胞的假说，我们在大鼠的骨骼肌挤压伤后检查了冰敷对 MCP-1+细胞和中性粒细胞的影响。由于损伤后 20 分钟内进行了冰敷，直到损伤后 12 小时，巨噬细胞的积累才受到抑制。损伤后 3 小时的中性粒细胞数量和损伤后 6 小时及以后的 MCP-1+细胞数量在冰敷组中明显低于非冰敷组，这表明这些现象导致了巨噬细胞迁移的延迟。
