Department of Conservative Dentistry and Periodontology, University Hospital, LMU Munich, Germany; Walther Straub Institute of Pharmacology and Toxicology, Faculty of Medicine, LMU Munich, Germany.
Institute of Anatomy, Neuroanatomy, Faculty of Medicine, LMU Munich, Germany.
Dent Mater. 2020 Mar;36(3):402-412. doi: 10.1016/j.dental.2020.01.013. Epub 2020 Jan 25.
Titanium (Ti)- and Zirconia (ZrO)-implants in mini pig maxillae were compared with respect to Ti/zirconium (Zr) release into the surrounding bone tissues, the resulting short term tissue responses and the potential toxicity.
Ti/Zr release from Ti- and ZrO-implants in mini pig maxillae was determined with inductively coupled plasma optical emission spectrometry (ICP-OES) and inductively coupled plasma mass spectrometry (ICP-MS). The spatial distribution of Ti and Zr in maxilla tissues near the implant surface was assessed with laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS). A histological analysis was performed to investigate the tissue responses after 12 weeks of implantation. The cytotoxicity and DNA damage of Ti particles and ZrO particles were studied with XTT and Comet assay.
The mean Ti content in the bone adjacent to Ti-implants was 1.67 mg/kg-bone weight. The highest Ti content detected was 2.17 mg/kg-bone weight. The mean Zr content in the bone adjected to ZrO-implants was 0.59 mg/kg-bone weight. The highest Zr content was 0.75 mg/kg-bone weight. The spatial distribution of the Ti and Zr in bone showed mainly a higher intensity of Ti and Zr close to the screw thread outer tip rather. Histological analysis indicated that near both implant-types signs of bone marrow fibrosis were present. EC of commercially available ZrO-nanoparticles (NPs, <100 nm) and ZrO-microparticles (MPs, <5 μm) was 13.96 mg/ml and 80.99 mg/ml, respectively. ZrO-NPs and ZrO-MPs can induce DNA damage at 70 μg/ml and 810 μg/ml, respectively.
After 12-weeks of implantation, increased concentrations of Ti and Zr can be detected in bone/tissues near Ti- and ZrO-implants in mini pig maxillae. Ti content released from Ti-implants is two times higher than the Zr content released from ZrO-implants. ZrO-NPs showed lower cytotoxicity and DNA damage compared to results reported for Ti-NPs in human cells.
比较钛（Ti）和氧化锆（ZrO）植入物在小型猪上颌骨中的情况，评估 Ti/Zr 释放到周围骨组织中的情况、短期组织反应和潜在毒性。
通过电感耦合等离子体发射光谱法（ICP-OES）和电感耦合等离子体质谱法（ICP-MS）测定 Ti 和 ZrO 植入物在小型猪上颌骨中的 Ti/Zr 释放情况。采用激光烧蚀-电感耦合等离子体质谱法（LA-ICP-MS）评估植入物表面附近上颌骨组织中 Ti 和 Zr 的空间分布。通过组织学分析研究植入 12 周后的组织反应。通过 XTT 和彗星试验研究 Ti 颗粒和 ZrO 颗粒的细胞毒性和 DNA 损伤。
Ti 植入物周围骨组织中 Ti 的平均含量为 1.67mg/kg 骨重，检测到的最高 Ti 含量为 2.17mg/kg 骨重。ZrO 植入物周围骨组织中 Zr 的平均含量为 0.59mg/kg 骨重，检测到的最高 Zr 含量为 0.75mg/kg 骨重。Ti 和 Zr 在骨中的空间分布主要表现为靠近螺钉螺纹外端的 Ti 和 Zr 强度较高。组织学分析表明，两种植入物附近均有骨髓纤维化的迹象。商业 ZrO 纳米颗粒（NPs，<100nm）和 ZrO 微米颗粒（MPs，<5μm）的 EC 值分别为 13.96mg/ml 和 80.99mg/ml。ZrO-NPs 和 ZrO-MPs 分别在 70μg/ml 和 810μg/ml 时可诱导 DNA 损伤。
在小型猪上颌骨植入后 12 周，可在 Ti 和 ZrO 植入物附近的骨/组织中检测到 Ti 和 Zr 浓度增加。Ti 植入物释放的 Ti 含量是 ZrO 植入物释放的 Zr 含量的两倍。与人类细胞中报道的 Ti-NPs 相比，ZrO-NPs 显示出较低的细胞毒性和 DNA 损伤。
