豚草叶绿体微卫星标记的开发（）。 （括号部分原文缺失具体内容）
Development of chloroplast microsatellite markers for giant ragweed ().
作者信息
Sharma Himanshu, Hyvönen Jaakko, Poczai Péter
机构信息
Agri-Biotechnology Divison National Agri-Food Biotechnology Institute Mohali 140306 India.
Finnish Museum of Natural History (Botany) University of Helsinki P.O. Box 7 Helsinki FI-00014 Finland.
出版信息
Appl Plant Sci. 2020 Jan 22;8(1):e11313. doi: 10.1002/aps3.11313. eCollection 2020 Jan.
PREMISE
Plant invasions are increasing globally, and extensive study of the genetic background of the source and invading populations is needed to understand such biological processes. For this reason, chloroplast microsatellite markers were identified to explore the genetic diversity of the noxious weed (Asteraceae).
METHODS AND RESULTS
The complete chloroplast genome of was mined for microsatellite loci, and 15 novel chloroplast primers were identified to assess the genetic diversity of 49 samples. The number of alleles amplified ranged from two to six, with an average of 3.2 alleles per locus. Shannon's information index varied from 0.305 and 1.467, expected heterozygosity ranged from 0.178 to 0.645, and the polymorphism information content value ranged from 0.211 to 0.675 (average 0.428). The cross-species transferability of the 15 microsatellite loci was also evaluated in four related species (, , , and ).
CONCLUSIONS
The novel chloroplast microsatellite markers developed in the current study demonstrate substantial cross-species transferability and will be helpful in future genetic diversity studies of and related species.
前提
植物入侵在全球范围内不断增加，需要对来源种群和入侵种群的遗传背景进行广泛研究，以了解此类生物过程。因此，鉴定了叶绿体微卫星标记，以探索这种有害杂草（菊科）的遗传多样性。
方法与结果
从该植物的完整叶绿体基因组中挖掘微卫星位点，鉴定出15对新的叶绿体引物，用于评估49个样本的遗传多样性。扩增出的等位基因数量从2个到6个不等，每个位点平均有3.2个等位基因。香农信息指数在0.305至1.467之间变化，期望杂合度在0.178至0.645之间，多态性信息含量值在0.211至0.675之间（平均0.428）。还在四个相关物种（[物种名称1]、[物种名称2]、[物种名称3]和[物种名称4]）中评估了这15个微卫星位点的种间转移性。
结论
本研究中开发的新型叶绿体微卫星标记显示出显著的种间转移性，将有助于未来对该植物及相关物种的遗传多样性研究。
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