Nagy Erzsébet, Hegedűs Géza, Taller János, Kutasy Barbara, Virág Eszter
University of Pannonia, Georgikon Faculty, Department of Plant Science and Biotechnology, Keszthely, Hungary.
University of Pannonia, Georgikon Faculty, Department of Economic Methodology, Keszthely, Hungary.
Data Brief. 2017 Oct 7;15:606-611. doi: 10.1016/j.dib.2017.10.009. eCollection 2017 Dec.
Common ragweed L. is the most widespread weed and the most dangerous pollen allergenic plant in large areas of the temperate zone. Since herbicides like PSI and PSII inhibitors have their target genes in the chloroplast genome, understanding the chloroplast genome may indirectly support the exploration of herbicide resistance and development of novel control methods. The aim of the present study was to sequence and reconstruct for the chloroplast genome of and establish a molecular dataset. We used an Illumina MiSeq protocol to sequence the chloroplast genome of isolated intact organelles of ragweed plants grown in our experimental garden. The assembled chloroplast genome was found to be 152,215 bp (GC: 37.6%) in a quadripartite structure, where 80 protein coding genes, 30 tRNA and 4 rRNA genes were annotated in total. We also report the complete sequence of 114 genes encoded in chloroplast genome supported by both MIRA and Velvet assemblers and ordered to L. using the Geneious software.
普通豚草是温带地区大面积分布最广的杂草和最危险的花粉致敏植物。由于PSI和PSII抑制剂等除草剂的靶基因位于叶绿体基因组中，了解叶绿体基因组可能间接有助于探索抗除草剂特性并开发新的防治方法。本研究的目的是对普通豚草的叶绿体基因组进行测序和重建，并建立一个分子数据集。我们使用Illumina MiSeq方案对在我们实验园中种植的豚草植物分离出的完整细胞器的叶绿体基因组进行测序。组装后的叶绿体基因组呈四分体结构，长度为152,215 bp（GC含量：37.6%），共注释了80个蛋白质编码基因、30个tRNA基因和4个rRNA基因。我们还报告了由MIRA和Velvet组装器支持并使用Geneious软件排列到普通豚草上的叶绿体基因组中编码的114个基因的完整序列。
