Bander S A, Watson S C, Shire J G
Department of Biology, University of Essex, Colchester, U.K.
J Reprod Fertil. 1988 Nov;84(2):709-14. doi: 10.1530/jrf.0.0840709.
The cumulus oophorus surrounding eggs from C57BL/6 mice was digested by bovine or leech hyaluronidase significantly more rapidly than that surrounding eggs from BALB/c mice. The zona pellucida of C57BL/6 eggs was also more rapidly attacked by pronase. Three other sublines of C57BL showed the same characteristics. Measurements of susceptibility to hyaluronidase and pronase on eggs from the CXB recombinant inbred strains indicated that variation at a minimum of 2 loci affected each character. The lack of correlation between susceptibilities to the 2 enzymes across the recombinant strains implied that these differences separately affect the substrates of the enzymes, rather than reflecting a common difference in the process of oocyte maturation. The variation in susceptibility was unrelated to differences, controlled by the Ped and Qa-2 loci, in the rate of later embryonic cleavage. However, pronase susceptibility was significantly correlated with the early onset of the first cleavage.
与BALB/c小鼠的卵周围的卵丘相比，来自C57BL/6小鼠的卵周围的卵丘被牛或水蛭透明质酸酶消化的速度明显更快。C57BL/6卵的透明带也更容易被链霉蛋白酶攻击。C57BL的其他三个亚系也表现出相同的特征。对CXB重组近交系的卵对透明质酸酶和链霉蛋白酶的敏感性测量表明，至少2个位点的变异影响了每个特征。在重组品系中，对这两种酶的敏感性之间缺乏相关性，这意味着这些差异分别影响酶的底物，而不是反映卵母细胞成熟过程中的共同差异。敏感性的变化与由Ped和Qa - 2位点控制的后期胚胎分裂速度的差异无关。然而，链霉蛋白酶敏感性与第一次分裂的早期开始显著相关。
