Korean Collection for Type Cultures, Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup, 56212, Republic of Korea.
Seoul National University Bundang Hospital, Seongnam, 13620, Republic of Korea.
J Microbiol. 2020 Feb;58(2):99-104. doi: 10.1007/s12275-020-9396-9. Epub 2020 Jan 29.
An obligately anaerobic, Gram-stain-negative, non-motile, non-spore-forming, and coccobacilli-shaped bacterial strain, designated KGMB03119, was isolated from human faeces from a Korean. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that the isolate was a member of the genus Sutterella and most closely related to Sutterlla wadsworthensis KCTC 15691 (96.8% 16S rRNA gene sequence similarity). The DNA G + C content of strain KGMB03119 was 58.3 mol% as determined from its whole genome sequence. Strain KGMB03119 was asaccharolytic, catalase-positive, oxidase- and urease-negative. Furthermore, the isolate was positive for alkaline phosphatase, leucine arylamidase, acid phosphatase, arginine arylamidase, alanine arylamidase, and glycine arylamidase. The major cellular fatty acids (> 10%) of the isolate were Cω9c and C. Methylmenaquinone-5 (MMK-5, 100%) was the predominant isoprenoid quinone in the isolate. Based on the phylogenetic, physiological, and chemotaxonomic characteristics, strain KGMB03119 represents a novel species, for which the name Sutterella faecalis sp. nov. is proposed. The type strain is KGMB03119 (= KCTC 15823 = NBRC 114254).
一株严格厌氧、革兰氏染色阴性、无动力、不产芽孢、呈球杆菌状的细菌菌株，被命名为 KGMB03119，从一位韩国人的粪便中分离得到。基于 16S rRNA 基因序列的系统发育分析表明，该分离株是 Sutterella 属的成员，与 Sutterella wadsworthensis KCTC 15691（96.8% 16S rRNA 基因序列相似性）最为密切相关。根据全基因组序列，菌株 KGMB03119 的 DNA G+C 含量为 58.3 mol%。菌株 KGMB03119 是一种非发酵糖的，过氧化氢酶阳性，氧化酶和脲酶阴性。此外，该分离株碱性磷酸酶、亮氨酸芳基酰胺酶、酸性磷酸酶、精氨酸芳基酰胺酶、丙氨酸芳基酰胺酶和甘氨酸芳基酰胺酶均为阳性。该分离株的主要细胞脂肪酸（>10%）为 Cω9c，甲基萘醌-5（MK-5，100%）是该分离株中主要的异戊二烯醌。根据系统发育、生理和化学分类学特征，菌株 KGMB03119 代表了一个新的物种，建议将其命名为 Sutterella faecalis sp. nov.。其模式菌株为 KGMB03119（=KCTC 15823=NBRC 114254）。
