1Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Research Center, Tsukuba, Ibaraki 305-0074, Japan.
2PRIME, Japan Agency for Medical Research and Development (AMED), Tsukuba, Ibaraki 305-0074, Japan.
Int J Syst Evol Microbiol. 2018 Dec;68(12):3942-3950. doi: 10.1099/ijsem.0.003096. Epub 2018 Nov 5.
Two novel, obligately anaerobic, Gram-stain-negative, rod or coccoid-shaped bacteria, designated strains 4NBBH2 and 6FBBBH3, were isolated from faecal samples of a healthy Japanese woman and man. The 16S rRNA gene sequence analysis showed that these strains represent a distinct lineage within the family Sutterellaceae. Strain 4NBBH2 formed a monophyletic branch between the genera Parasutterella and Sutterella, with sequence similarity to Sutterella stercoricanis CCUG 47620 (92.6 %), followed by Sutterella wadsworthensis WAL 7877 (92.4 %), Sutterella parvirubra YIT 11816 (92.1 %) and Parasutterella secunda YIT 12071 (91.8 %). Strain 6FBBBH3 was affiliated to the genus Sutterella, with highest similarity to S. stercoricanis CCUG 47620 (97.1 %), followed by S. parvirubra YIT 11816 (96.6 %) and S. wadsworthensis WAL 7877 (95.2 %). Strains 4NBBH2 and 6FBBBH3 were asaccharolytic. Analysis of fatty acids revealed that strain 4NBBH2 could be differentiated from Sutterella species (including strain 6FBBBH3) by the presence of a low concentration of C16 : 1ω7c. The major respiratory quinones of strain 4NBBH2 were menaquinone (MK)-6 and methylmenaquinone (MMK)-6, whereas those of strain 6FBBBH3 were MK-5 and MMK-5. The G+C content of the genomic DNA of strains 4NBBH2 and 6FBBBH3 were 56.9 and 62.8 mol%, respectively. On the basis of the collected data, strain 4NBBH2 represents a novel species in a novel genus of the family Sutterellaceae, for which the name Mesosutterella multiformis gen. nov., sp. nov. is proposed. The type strain is 4NBBH2 (=JCM 32464=DSM 106860). We also propose a novel Sutterella species, Sutterellamegalosphaeroides sp. nov., for strain 6FBBBH3 (=JCM 32470=DSM 106861).
从一名健康的日本女性和男性的粪便样本中分离到两株新型严格厌氧菌，革兰氏染色阴性，呈杆状或球状的细菌，分别命名为菌株 4NBBH2 和 6FBBBH3。16S rRNA 基因序列分析表明，这些菌株在 Sutterellaceae 科内代表一个独特的谱系。菌株 4NBBH2 在 Parasutterella 和 Sutterella 属之间形成一个单系分支，与 Sutterella stercoricanis CCUG 47620（92.6%）的序列相似性最高，其次是 Sutterella wadsworthensis WAL 7877（92.4%）、Sutterella parvirubra YIT 11816（92.1%）和 Parasutterella secunda YIT 12071（91.8%）。菌株 6FBBBH3 属于 Sutterella 属，与 Sutterella stercoricanis CCUG 47620（97.1%）的相似度最高，其次是 Sutterella parvirubra YIT 11816（96.6%）和 Sutterella wadsworthensis WAL 7877（95.2%）。菌株 4NBBH2 和 6FBBBH3 为非发酵菌。脂肪酸分析表明，与 Sutterella 属（包括菌株 6FBBBH3）相比，菌株 4NBBH2 可以通过存在低浓度的 C16:1ω7c 来区分。菌株 4NBBH2 的主要呼吸醌为 Menaquinone（MK）-6 和 Methylmenaquinone（MMK）-6，而菌株 6FBBBH3 的主要呼吸醌为 MK-5 和 MMK-5。菌株 4NBBH2 和 6FBBBH3 的基因组 DNA G+C 含量分别为 56.9%和 62.8%mol。基于收集的数据，菌株 4NBBH2 代表 Sutterellaceae 科中一个新型属的新型物种，提议将其命名为 Mesosutterella multiformis gen. nov.，sp. nov.。模式菌株为 4NBBH2（=JCM 32464=DSM 106860）。我们还提议为菌株 6FBBBH3（=JCM 32470=DSM 106861）创建一个新的 Sutterella 物种，即 Sutterellamegalosphaeroides sp. nov.。
