Korean Collection for Type Cultures, Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup 56212, Republic of Korea.
National Institute of Animal Science, Cheonan 31000, Republic of Korea.
Int J Syst Evol Microbiol. 2021 Jun;71(6). doi: 10.1099/ijsem.0.004836.
An obligately anaerobic, Gram-positive, non-motile, coccus-shaped bacterial strain designated AGMB00490 was isolated from swine faeces. 16S rRNA gene sequence-based phylogenetic analysis indicated that the isolate belongs to the genus and that the most closely related species is WAL 10418 (=KCTC 5947, 97.22 % 16S rRNA gene sequence similarity). Whole genome sequence analysis determined that the DNA G+C content of strain AGMB00490 was 31.2 mol% and moreover that the genome size and numbers of tRNA and rRNA genes were 2 129 517 bp, 34 and 10, respectively. Strain AGMB00490 was negative for oxidase and urease; positive for catalase, indole production, arginine arylamidase, leucine arylamidase, tyrosine arylamidase and histidine arylamidase; and weakly positive for phenylalanine arylamidase and glycine arylamidase. The major cellular fatty acids (>10 %) of the isolate were determined to be C and C ω9. Strain AGMB00490 produced acetic acid as a major end product of metabolism. Accordingly, phylogenetic, physiologic and chemotaxonomic analyses revealed that strain AGMB00490 represents a novel species for which the name sp. nov. is proposed. The type strain is AGMB00490 (=KCTC 15944=NBRC 114159).
从猪粪便中分离到一株严格厌氧、革兰氏阳性、非运动、球状的细菌菌株，命名为 AGMB00490。基于 16S rRNA 基因序列的系统发育分析表明，该分离株属于 属，与最密切相关的物种是 WAL 10418（=KCTC 5947，16S rRNA 基因序列相似性为 97.22%）。全基因组序列分析确定菌株 AGMB00490 的 DNA G+C 含量为 31.2mol%，基因组大小和 tRNA 和 rRNA 基因的数量分别为 2129517bp、34 和 10。菌株 AGMB00490 氧化酶和脲酶均为阴性；过氧化氢酶、吲哚产生、精氨酸芳基酰胺酶、亮氨酸芳基酰胺酶、酪氨酸芳基酰胺酶和组氨酸芳基酰胺酶均为阳性；苯丙氨酸芳基酰胺酶和甘氨酸芳基酰胺酶为弱阳性。该分离株的主要细胞脂肪酸（>10%）为 C 和 Cω9。菌株 AGMB00490 以乙酸为主要代谢终产物。因此，系统发育、生理和化学分类学分析表明，菌株 AGMB00490 代表一个新的种，提议命名为 sp. nov.。其模式菌株为 AGMB00490（=KCTC 15944=NBRC 114159）。
